PDGF R beta Antibody (2G7B7) - BSA Free

Novus Biologicals | Catalog # NBP2-52529

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot, ELISA, Flow Cytometry, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 2G7B7

Format

BSA Free
View all PDGF R beta Primary Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human PDGF R beta (AA: 33-133) expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

124 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PDGF R beta Antibody (2G7B7) - BSA Free

Western Blot: PDGF R beta Antibody (2G7B7)BSA Free [NBP2-52529]

Western Blot: PDGF R beta Antibody (2G7B7)BSA Free [NBP2-52529]

Western Blot: PDGF R beta Antibody (2G7B7) [NBP2-52529] - Analysis using PDGFRB mAb against HEK293 (1) and PDGFRB (AA: 33-133)-hIgGFc transfected HEK293 (2) cell lysate.
Flow Cytometry: PDGF R beta Antibody (2G7B7) - BSA Free [NBP2-52529]

Flow Cytometry: PDGF R beta Antibody (2G7B7) - BSA Free [NBP2-52529]

Flow Cytometry: PDGF R beta Antibody (2G7B7) [NBP2-52529] - Analysis of NIH/3T3 cells using PDGFRB mouse mAb (green) and negative control (red).
Western Blot: PDGF R beta Antibody (2G7B7)BSA Free [NBP2-52529]

Western Blot: PDGF R beta Antibody (2G7B7)BSA Free [NBP2-52529]

Western Blot: PDGF R beta Antibody (2G7B7) [NBP2-52529] - Analysis using PDGFRB mAb against human PDGFRB (AA: 33-133) recombinant protein. (Expected MW is 37 kDa).
ELISA: PDGF R beta Antibody (2G7B7) - BSA Free [NBP2-52529]

ELISA: PDGF R beta Antibody (2G7B7) - BSA Free [NBP2-52529]

ELISA: PDGF R beta Antibody (2G7B7) [NBP2-52529] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng)

Applications for PDGF R beta Antibody (2G7B7) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200-1:400

Western Blot

1:500-1:2000
Application Notes
This antibody is Cytof ready.

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PDGF R beta

Human platelet-derived growth factor (PDGF) receptor, is a receptor tyrosine kinase whose activity induces various signalling cascades leading to cell growth and proliferation. Two major isoforms exist, PDGF receptor-alpha and PDGF receptor-beta. PDGF receptor-beta can only bind two specific PDGF isoforms, PDGF-beta and PDGF-delta (1,2). Upon ligand binding, the receptor undergoes autophosphorylation, allowing binding and activation of cytoplasmic SH2 domain-containing signal transduction molecules such as PI kinase (3), Grb2, and Src, among others.

Long Name

Platelet-derived Growth Factor Receptor beta

Alternate Names

CD140b, PDGFRB

Gene Symbol

PDGFRB

Additional PDGF R beta Products

Product Documents for PDGF R beta Antibody (2G7B7) - BSA Free

Certificate of Analysis

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Product Specific Notices for PDGF R beta Antibody (2G7B7) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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