PDGF R beta Antibody (APB5) - BSA Free

Novus Biologicals | Catalog # NBP1-43349

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Frozen, Flow Cytometry, Protein Array

Cited:

Immunohistochemistry-Frozen, Flow Cytometry, Protein Array

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2a Kappa Clone # APB5

Format

BSA Free
View all PDGF R beta Primary Antibodies »

Product Specifications

Immunogen

The immunogen for this antibody was PDGF receptor beta.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2a Kappa

Scientific Data Images for PDGF R beta Antibody (APB5) - BSA Free

Flow Cytometry: PDGF R beta Antibody (APB5) - BSA Free [NBP1-43349]

Flow Cytometry: PDGF R beta Antibody (APB5) - BSA Free [NBP1-43349]

Flow Cytometry: PDGF R beta Antibody (APB5) [NBP1-43349] - Mouse brain pericytes - IgG (left) and PDGFRB (right). Image from verified customer review.
Flow Cytometry: PDGF R beta Antibody (APB5) - BSA Free [NBP1-43349]

Flow Cytometry: PDGF R beta Antibody (APB5) - BSA Free [NBP1-43349]

Flow Cytometry: PDGF R beta Antibody (APB5) [NBP1-43349] - Staining of NIH/3T3 cell line with 0.5 ug of Rat IgG2a kappa Isotype Control Purified (cat. 14-4321) (open histogram) or 0.5 ug of Anti-Mouse CD140b (PDGF Receptor b) Purified (filled histogram) followed by Anti-Rat IgG Biotin (cat. 13-4813) and Streptavidin PE (cat. 12-4317). Total viable cells were used for analysis.
Flow Cytometry: PDGF R beta Antibody (APB5) - BSA Free [NBP1-43349]

Flow Cytometry: PDGF R beta Antibody (APB5) - BSA Free [NBP1-43349]

Flow Cytometry: PDGF R beta Antibody (APB5) [NBP1-43349] - Analysis using the Biotin conjugate of NBP1-43349. Staining of NIH/3T3 cell line with 0.5 ug of Anti-Mouse CD140b (PDGF Receptor b) Biotin followed by Streptavidin PE. Appropriate isotype controls were used (open histogram).

Applications for PDGF R beta Antibody (APB5) - BSA Free

Application
Recommended Usage

Flow Cytometry

< 1.0 ug/10^6 cells

Immunohistochemistry

1:10 - 1:500

Immunohistochemistry-Frozen

1:10 - 1:500
Application Notes
The APB5 antibody has been tested by flow cytometric analysis of NIH-3T3 cells. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Use in Protein Array reported in secitific publication PMID: 32499572

Reviewed Applications

Read 1 review rated 5 using NBP1-43349 in the following applications:

Flow Cytometry Panel Builder

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  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS (pH 7.2)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: PDGF R beta

The APB5 monoclonal antibody reacts with the mouse CD140b molecule, the beta chain of the platelet derived growth factor receptor (PDGF receptor). PDGFRb is a receptor tyrosine kinase that forms dimers on the surface upon ligand binding and phosphorylates substrates. Dimers of PDGFR consist of either homodimers of alpha/alpha, beta/beta, or heterodimers of alpha/beta and serve as a substrate for its kinase activity. CD140b is expressed by embryonic tissues and mesenchymal-derived cells of the adult mouse tissues. The PDGFR beta chain is reported to play a significant role in formation of fibrous atherosclerotic lesions.

Long Name

Platelet-derived Growth Factor Receptor beta

Alternate Names

CD140b, PDGFRB

Entrez Gene IDs

18596 (Mouse)

Gene Symbol

PDGFRB

UniProt

P05622

Additional PDGF R beta Products

Product Documents for PDGF R beta Antibody (APB5) - BSA Free

Certificate of Analysis

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Product Specific Notices for PDGF R beta Antibody (APB5) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for PDGF R beta Antibody (APB5) - BSA Free

Customer Reviews for PDGF R beta Antibody (APB5) - BSA Free (1)

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Customer Images

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  • PDGF R beta Antibody (APB5)
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: Brain pericytes
    Species: Mouse
    Verified Customer | Posted 11/12/2018
    FACS for mouse brain pericytes - IgG (left) and PDGFRB (right)
    Mouse brain pericytes were examined for PDGFRB surface expression
    PDGF R beta Antibody (APB5) - BSA Free NBP1-43349

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