PDGFR alpha Antibody (16A1) - BSA Free

Novus Biologicals | Catalog # NBP1-44581

Clone 16A1 was used by HLDA to establish CD designation.
Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Flow Cytometry, Flow (Cell Surface), Immunocytochemistry/ Immunofluorescence, In vitro assay, CyTOF-ready

Cited:

In vitro assay

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 16A1

Format

BSA Free
View all PDGF R alpha Primary Antibodies »

Product Specifications

Immunogen

CD140a-transfected NIH 3T3 cells

Specificity

The mouse monoclonal antibody 16A1 recognizes CD140a / PDGF-RA, the 170 kDa alpha chain of platelet-derived growth factor receptor, which is widely expressed on a variety of mesenchymal-derived cells and plays pro-proliferative or anti-proliferative roles in various tumours.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

170 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PDGFR alpha Antibody (16A1) - BSA Free

Immunocytochemistry/ Immunofluorescence: PDGFR alpha Antibody (16A1) - BSA Free [NBP1-44581]

Immunocytochemistry/ Immunofluorescence: PDGFR alpha Antibody (16A1) - BSA Free [NBP1-44581]

PDGFR-alpha-Antibody-16A1-Immunocytochemistry-Immunofluorescence-NBP1-44581-img0006.jpg
Flow (Cell Surface): PDGFR alpha Antibody (16A1) - BSA Free [NBP1-44581]

Flow (Cell Surface): PDGFR alpha Antibody (16A1) - BSA Free [NBP1-44581]

Flow (Cell Surface): PDGFR alpha Antibody (16A1) [NBP1-44581] - Surface staining of CD140a in CD140a-transfected cells using anti-CD140a (16A1) purified / GAM-APC.
Flow Cytometry: PDGFR alpha Antibody (16A1) - BSA Free [NBP1-44581]

Flow Cytometry: PDGFR alpha Antibody (16A1) - BSA Free [NBP1-44581]

Flow Cytometry: PDGFR alpha Antibody (16A1) [NBP1-44581] - CD140a in CD140a-transfected 3T3 cells using anti-CD140a (16A1) APC.
Flow Cytometry: PDGFR alpha Antibody (16A1) - BSA Free [NBP1-44581]

Flow Cytometry: PDGFR alpha Antibody (16A1) - BSA Free [NBP1-44581]

Flow Cytometry: PDGFR alpha Antibody (16A1) [NBP1-44581] - CD140a in CD140a-transfected cells using anti-CD140a (16A1) PE.
Flow (Cell Surface): PDGFR alpha Antibody (16A1) - BSA Free [NBP1-44581]

Flow (Cell Surface): PDGFR alpha Antibody (16A1) - BSA Free [NBP1-44581]

Flow (Cell Surface): PDGFR alpha Antibody (16A1) [NBP1-44581] - Surface staining of CD140a in CD140a-transfected cells using anti-CD140a (16A1) FITC.
Flow (Cell Surface): PDGFR alpha Antibody (16A1) - BSA Free [NBP1-44581]

Flow (Cell Surface): PDGFR alpha Antibody (16A1) - BSA Free [NBP1-44581]

Flow (Cell Surface): PDGFR alpha Antibody (16A1) [NBP1-44581] - Surface staining of CD140a in CD140a-transfected cells using anti-CD140a (16A1) biotin / streptavidin-APC.

Applications for PDGFR alpha Antibody (16A1) - BSA Free

Application
Recommended Usage

Flow Cytometry

2 ug/ml

Immunocytochemistry/ Immunofluorescence

1:10-1:2000
Application Notes
This antibody is Cytof ready. Use in In vitro reported in scientific literature (PMID: 23028311).

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

Phosphate buffered saline (PBS), pH 7.4

Format

BSA Free

Preservative

15mM Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: PDGF R alpha

CD140a / PDGF-RA (platelet-derived growth factor receptor alpha) is a cell surface receptor for members of platelet-derived growth factor family, whose intracellular part contains a tyrosine kinase domain. CD140a forms homodimers, or heterodimerizes with CD140b / PDGF-RB. Whereas CD140b induces in different cell types their proliferation and migration, the role of CD140a is more controversial, with pro-proliferative or anti-proliferative effects. CD140a has early developmental functions, mediates mesodermal cell migration, and later acts in signaling associated in epithelial-mesenchymal interactions.

Long Name

Platelet-derived Growth Factor Receptor alpha

Alternate Names

CD140a, PDGFR alpha, PDGFRA

Gene Symbol

PDGFRA

Additional PDGF R alpha Products

Product Documents for PDGFR alpha Antibody (16A1) - BSA Free

Certificate of Analysis

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Product Specific Notices for PDGFR alpha Antibody (16A1) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for PDGFR alpha Antibody (16A1) - BSA Free

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Protocols

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