PDGFR alpha Antibody (16A1) [Biotin]
Novus Biologicals | Catalog # NBP1-47239
Clone 16A1 was used by HLDA to establish CD designation.
Key Product Details
Species Reactivity
Human
Applications
Flow Cytometry, Flow (Cell Surface), Immunocytochemistry/ Immunofluorescence
Label
Biotin
Antibody Source
Monoclonal Mouse IgG1 Clone # 16A1
Loading...
Product Specifications
Immunogen
CD140a-transfected NIH 3T3 cells
Specificity
The mouse monoclonal antibody 16A1 recognizes CD140a / PDGF-RA, the 170 kDa alpha chain of platelet-derived growth factor receptor, which is widely expressed on a variety of mesenchymal-derived cells and plays pro-proliferative or anti-proliferative roles in various tumours.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
170 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Store in the dark. Avoid prolonged exposure to light.
Scientific Data Images for PDGFR alpha Antibody (16A1) [Biotin]
Flow (Cell Surface): PDGF R alpha Antibody (16A1) [Biotin] [NBP1-47239] - Surface staining of CD140a in CD140a-transfected cells using anti-CD140a (16A1) purified / GAM-APC.
Flow (Cell Surface): PDGF R alpha Antibody (16A1) [Biotin] [NBP1-47239] - Surface staining of CD140a in CD140a-transfected 3T3 cells using anti-CD140a (16A1) APC.
Flow (Cell Surface): PDGF R alpha Antibody (16A1) [Biotin] [NBP1-47239] - Surface staining of CD140a in CD140a-transfected cells using anti-CD140a (16A1) PE.
Flow (Cell Surface): PDGF R alpha Antibody (16A1) [Biotin] [NBP1-47239] - Surface staining of CD140a in CD140a-transfected cells using anti-CD140a (16A1) FITC.
Flow (Cell Surface): PDGF R alpha Antibody (16A1) [Biotin] [NBP1-47239] - Surface staining of CD140a in CD140a-transfected cells using anti-CD140a (16A1) biotin / streptavidin-APC.
Applications for PDGFR alpha Antibody (16A1) [Biotin]
Application
Recommended Usage
Flow Cytometry
1-2 ug/ml
Immunocytochemistry/ Immunofluorescence
1:10-1:2000
Flow Cytometry Panel Builder
Bio-Techne Knows Flow Cytometry
Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.
Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
Phosphate buffered saline (PBS), pH 7.4
Preservative
15mM Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: PDGF R alpha
Long Name
Platelet-derived Growth Factor Receptor alpha
Alternate Names
CD140a, PDGFR alpha, PDGFRA
Gene Symbol
PDGFRA
Additional PDGF R alpha Products
Product Documents for PDGFR alpha Antibody (16A1) [Biotin]
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for PDGFR alpha Antibody (16A1) [Biotin]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Customer Reviews for PDGFR alpha Antibody (16A1) [Biotin]
There are currently no reviews for this product. Be the first to review PDGFR alpha Antibody (16A1) [Biotin] and earn rewards!
Have you used PDGFR alpha Antibody (16A1) [Biotin]?
Submit a review and receive an Amazon gift card!
$25/€18/£15/$25CAN/¥2500 Yen for a review with an image
$10/€7/£6/$10CAN/¥1110 Yen for a review without an image
Submit a review
Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- 7-Amino Actinomycin D (7-AAD) Cell Viability Flow Cytometry Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Extracellular Membrane Flow Cytometry Protocol
- Flow Cytometry Protocol for Cell Surface Markers
- Flow Cytometry Protocol for Staining Membrane Associated Proteins
- Flow Cytometry Staining Protocols
- Flow Cytometry Troubleshooting Guide
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Intracellular Flow Cytometry Protocol Using Alcohol (Methanol)
- Intracellular Flow Cytometry Protocol Using Detergents
- Intracellular Nuclear Staining Flow Cytometry Protocol Using Detergents
- Intracellular Staining Flow Cytometry Protocol Using Alcohol Permeabilization
- Intracellular Staining Flow Cytometry Protocol Using Detergents to Permeabilize Cells
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Propidium Iodide Cell Viability Flow Cytometry Protocol
- Protocol for Liperfluo
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Characterization of Human Th22 Cells
- Protocol for the Characterization of Human Th9 Cells
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
