PDZK1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-82572

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown, Orthogonal Validation

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all PDZK1 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: MTSTFNPRECKLSKQEGQNYGFFLRIEKDTEGHLVRVVEKCSPAEKAGLQDGDRVLRINGVFVDKEEHMQVVDLVRKSGNSVTLLVLDGDSYEKAV

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PDZK1 Antibody - BSA Free

Immunohistochemistry-Paraffin: PDZK1 Antibody [NBP1-82572]

Immunohistochemistry-Paraffin: PDZK1 Antibody [NBP1-82572]

Immunohistochemistry-Paraffin: PDZK1 Antibody [NBP1-82572] - Staining in human kidney and tonsil tissues.. Corresponding PDZK1 RNA-seq data are presented for the same tissues.
Western Blot: PDZK1 Antibody [NBP1-82572]

Western Blot: PDZK1 Antibody [NBP1-82572]

Western Blot: PDZK1 Antibody [NBP1-82572] - Analysis in Caco-2 cells transfected with control siRNA, target specific siRNA probe #1, using anti-PDZK1 antibody. Remaining relative intensity is presented. Loading control: anti-GAPDH.
Immunocytochemistry/ Immunofluorescence: PDZK1 Antibody [NBP1-82572]

Immunocytochemistry/ Immunofluorescence: PDZK1 Antibody [NBP1-82572]

Immunocytochemistry/Immunofluorescence: PDZK1 Antibody [NBP1-82572] - Staining of human cell line RPTEC TERT1 shows localization to nucleus, cytosol & vesicles. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: PDZK1 Antibody [NBP1-82572]

Immunohistochemistry-Paraffin: PDZK1 Antibody [NBP1-82572]

Immunohistochemistry-Paraffin: PDZK1 Antibody [NBP1-82572] - Staining of human tonsil shows no positivity as expected.
Western Blot: PDZK1 Antibody [NBP1-82572]

Western Blot: PDZK1 Antibody [NBP1-82572]

Western Blot: PDZK1 Antibody [NBP1-82572] - Western blot analysis in mouse liver tissue and rat liver tissue.
Immunohistochemistry-Paraffin: PDZK1 Antibody [NBP1-82572]

Immunohistochemistry-Paraffin: PDZK1 Antibody [NBP1-82572]

Immunohistochemistry-Paraffin: PDZK1 Antibody [NBP1-82572] - Staining of human pancreas shows weak to moderate positivity in plasma membrane in islets of Langerhans.
Immunohistochemistry-Paraffin: PDZK1 Antibody [NBP1-82572]

Immunohistochemistry-Paraffin: PDZK1 Antibody [NBP1-82572]

Immunohistochemistry-Paraffin: PDZK1 Antibody [NBP1-82572] - Staining of human small intestine shows moderate to strong positivity in luminal membrane in glandular cells.
Immunohistochemistry-Paraffin: PDZK1 Antibody [NBP1-82572]

Immunohistochemistry-Paraffin: PDZK1 Antibody [NBP1-82572]

Immunohistochemistry-Paraffin: PDZK1 Antibody [NBP1-82572] - Staining of human kidney shows moderate to strong positivity in apical membrane in cells in proximal tubules.

Applications for PDZK1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: PFA/Triton X-100

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PDZK1

PDZK1 is a scaffold protein that connects plasma membrane proteins and regulatory components, regulating their surface expression in epithelial cells apical domains. It may be involved in the coordination of a diverse range of regulatory processes for ion transport and second messenger cascades. In complex with SLC9A3R1, PDZK1 may cluster proteins that are functionally dependent in a mutual fashion and modulate the trafficking and the activity of the associated membrane proteins. It may also play a role in the cellular mechanisms associated with multidrug resistance through its interaction with ABCC2 and PDZK1IP1. PDZK1 may potentiate the CFTR chloride channel activity. PDZK1 functions to connect SCARB1 with the cellular machineries for intracellular cholesterol transport and/or metabolism and may be involved in the regulation of proximal tubular Na(+)-dependent inorganic phosphate cotransport, therefore playing an important role in tubule function.

Long Name

PDZ Domain Containing 1

Alternate Names

CAP70, CLAMP, NaPi-Cap1, NHERF3, PDZD1

Gene Symbol

PDZK1

Additional PDZK1 Products

Product Documents for PDZK1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for PDZK1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Epithelial Cell Polarity

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Protocols

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FAQs

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