PER2 Antibody - BSA Free

Novus Biologicals | Catalog # NB100-125

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse, Rat

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Cited:

Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all PER2 Primary Antibodies »

Product Specifications

Immunogen

A partial synthetic peptide made to an internal portion of the human PER2 protein (between amino acids 100-150) [UniProt# O15055].

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 30016666). Use in Rat reported in scientific literature (PMID:32119846).

Localization

Nuclear

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PER2 Antibody - BSA Free

Western Blot: PER2 AntibodyBSA Free [NB100-125]

Western Blot: PER2 AntibodyBSA Free [NB100-125]

Western Blot: PER2 Antibody [NB100-125] - Western Blot Usage of NB100-125. Image submitted via verified customer review.
Immunocytochemistry/ Immunofluorescence: PER2 Antibody - BSA Free [NB100-125]

Immunocytochemistry/ Immunofluorescence: PER2 Antibody - BSA Free [NB100-125]

Immunocytochemistry/Immunofluorescence: PER2 Antibody [NB100-125] - U-87 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated with anti-PER2 Antibody at 5 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Western Blot: PER2 AntibodyBSA Free [NB100-125]

Western Blot: PER2 AntibodyBSA Free [NB100-125]

Western Blot: PER2 Antibody [NB100-125] - WB analysis of PER2 expression on ARPE-19 whole cell lysate.
Immunocytochemistry/ Immunofluorescence: PER2 Antibody - BSA Free [NB100-125]

Immunocytochemistry/ Immunofluorescence: PER2 Antibody - BSA Free [NB100-125]

Immunocytochemistry/Immunofluorescence: PER2 Antibody [NB100-125] - The PER2 antibody was tested in ARPE-19 cells at a 1:250 dilution against Dylight 488 (Green). Alpha-tubulin and nuclei were counterstained against Dylight 550 (Red) and DAPI (Blue), respectively.
Immunocytochemistry/ Immunofluorescence: PER2 Antibody - BSA Free [NB100-125]

Immunocytochemistry/ Immunofluorescence: PER2 Antibody - BSA Free [NB100-125]

Immunocytochemistry/Immunofluorescence: PER2 Antibody [NB100-125] - MCF7 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated with anti-PER2 Antibody at 5 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
PER2 Antibody - BSA Free

Western Blot: PER2 Antibody - BSA Free [NB100-125] -

Deletion of IL-1 beta signaling ameliorates expression of hepatic circadian transcription factors following DSS-PN.8–10 week old IL1KO mice were subjected to Chow feeding or DSS-PN. A. Liver tissue was harvested, and mRNA expression of transcription factors regulating hepatic CR analyzed by qRT-PCR. Expression was normalized against HPRT. Values are Mean+/- SEM, N = at least 3/condition. *p<0.05. **p<0.01, ***P<0.001, ****P<0.0001. B. Western analysis of circadian regulatory proteins. Expression was normalized using Gapdh expression for each blot. Values are Mean+/- SEM, N = 3/condition, a = significantly different from respective chow control, b = significantly different compared to WT DSS-PN. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37647292), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
PER2 Antibody - BSA Free

Western Blot: PER2 Antibody - BSA Free [NB100-125] -

Genetic inhibition of TNF alpha signaling normalizes expression of hepatic circadian transcription factors following DSS-PN.8–10 week old TNFRKO mice were subjected to Chow feeding or DSS-PN. A. Liver tissue was harvested, and mRNA expression of transcription factors regulating hepatic CR analyzed by qRT-PCR. Expression was normalized against HPRT. N = 4 per condition. Values are Mean+/- SEM. *p<0.05. **p<0.01, ***p<0.001. B. Western analysis of circadian regulatory proteins. Expression was normalized using Gapdh expression for each blot. Values are Mean+/- SEM, N = 3/condition, a = significantly different from respective chow control, b = significantly different compared to WT DSS-PN. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37647292), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for PER2 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:500

Immunohistochemistry

1:200

Immunohistochemistry-Paraffin

1:200. Use reported in scientific literature (PMID 31822134)

Immunoprecipitation

reported in scientific literature (PMID 31390562)

Western Blot

1 - 2 ug/ml
Application Notes
This PER2 antibody is useful in Western blot and Immunocytochemistry/Immunofluorescence. It has been tested against expressed fragment of human PER2 (bacterially expressed fragment from BL21 cells), or full-length protein expressed in in vitro translation using rabbit reticulocyte lysate. In Western blot, a band can be seen at ~200 kDa representing PER2.

Reviewed Applications

Read 3 reviews rated 3.7 using NB100-125 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PER2

PER2 (period circadian protein homolog 2 or circadian clock protein PERIOD 2) is a component of the circadian clock mechanism which is essential for generating circadian rhythms and PER2 acts as a negative element in the circadian transcriptional loop. PER2 modulates the clock function by interacting with other circadian regulatory proteins for transporting them to nucleus, and negatively regulates CLOCK|NPAS2-BMAL1|BMAL2-induced transactivation. PER2 forms a component of the circadian core oscillator, which also includes the CRY proteins, CLOCK or NPAS2, BMAL1 or BMAL2, CSNK1D and/or CSNK1E, TIMELESS proteinsetc. PER2 interacts with NFIL3, PER1, PER3, CRY1 and CRY2 proteins, and its interaction with CSNK1D or CSNK1E promotes nuclear localization of PER proteins. PER2 is a nuclear protein and its nucleo-cytoplasmic shuttling is effected by interaction with other circadian core oscillator proteins and/or by phosphorylation. CSNK1E and CSNK1D facilitate PER2 phosphorylation that follows its degradation. PER2 defects have been found to be responsible for familial advanced sleep-phase syndrome (FASPS).

Alternate Names

Circadian clock protein PERIOD 2, FASPS, hPER2, KIAA0347period 2, period (Drosophila) homolog 2, period circadian protein 2, period circadian protein homolog 2, period homolog 2 (Drosophila)

Entrez Gene IDs

8864 (Human)

Gene Symbol

PER2

UniProt

O15055 (Human)

Additional PER2 Products

Product Documents for PER2 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for PER2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for PER2 Antibody - BSA Free

Customer Reviews for PER2 Antibody - BSA Free (3)

3.7 out of 5
3 Customer Ratings
5 Stars
33%
4 Stars
33%
3 Stars
0%
2 Stars
33%
1 Stars
0%

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Customer Images

Showing  1 - 3 of 3 reviews Showing All
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  • PER2 Antibody
    Name: Katharina Sahiri
    Application: Western Blot
    Sample Tested: U-2 OS cells
    Species: Human
    Verified Customer | Posted 02/21/2019
    siRNA transfection optimisation: whole cell lysate of U-2 OS cells a) siRNA knockdown of PER1 (load 25 µg/well) b) wt U-2 OS cells with different protein amounts/well 1°AB over night, 4 °C 2°AB 1h, RT Nitrocellulose Membrane0
    unspecific bands with no obvious band of correct size
    PER2 Antibody - BSA Free NB100-125
  • PER2 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Human cancer cell line
    Species: Human
    Verified Customer | Posted 11/12/2017
    Sample: Whole cell lysates of cancer cell lines (10ug) Blocking: 5% nonfat dry milk reconstituted in TBS-T for 1 hour at room temperature Primary Antibody diluted (1:500) in 5% nonfat dry milk reconstituted in TBS-T overnight at 4deg C Secondary Antibody: Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution in TBS-T
    PER2 Antibody - BSA Free NB100-125
  • Name: Anonymous
    Application: Western Blot
    Sample Tested: Whole cell lysates of various cancer cell lines
    Species: Human
    Verified Customer | Posted 12/10/2014
    PER2
    PER2 Antibody - BSA Free NB100-125

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Protocols

View specific protocols for PER2 Antibody - BSA Free (NB100-125):

PER2 Antibody:
Immunocytochemistry Protocol

Culture cells to appropriate density on suitable glass coverslips in 35 mm culture dishes or 6-well plates.

1. Remove culture medium and add 10% formalin to the dish. Fix at room temperature for 5-10 minutes.
2. Remove the formalin and add 0.5% Triton-X 100 in TBS to permeabilize the cells. Incubate for 5-10 minutes.
3. Remove the permeabilization buffer and add wash buffer (i.e. PBS or PBS with 0.1% Tween-20). Be sure to not let the specimen dry out. Gently wash three times for 10 minutes.
4. Alternatively, cells can be fixed with -20C methanol for 10 min at room temperature. Remove the methanol and rehydrate in PBS for 10 min before proceeding.
5. To block nonspecific antibody binding incubate in 10% normal goat serum for 1 hour at room temperature.
6. Add primary antibody at appropriate dilution and incubate at room temperature for 1 hour or at 4C overnight.
7. Remove primary antibody and replace with wash buffer. Gently wash three times for 10 minutes.
8. Add secondary antibody at the appropriate dilution. Incubate for 1 hour at room temperature.
9. Remove antibody and replace with wash buffer. Gently wash three times for 10 minutes.
10. Nuclei can be staining with 4',6' diamino phenylindole (DAPI) at 0.1 ug/ml, or coverslips can be directly mounted in media containing DAPI.
11. Cells can now be viewed with a fluorescence microscope.

*The above information is only intended as a guide. The researcher should determine what protocol best meets their needs. Please follow proper laboratory procedures for the disposal of formalin.

PER2 Antibody:
Western Blot Protocol
1. Perform SDS-PAGE on samples to be analyzed, loading 40 ug of total protein per lane.
2. Transfer proteins to membrane according to the instructions provided by the manufacturer of the membrane and transfer apparatus.
3. Stain according to standard Ponceau S procedure (or similar product) to assess transfer success, and mark molecular weight standards where appropriate.
4. Rinse the blot.
5. Block the membrane using standard blocking buffer for at least 1 hour.
6. Wash the membrane in wash buffer three times for 10 minutes each.
7. Dilute primary antibody in blocking buffer and incubate 1 hour at room temperature.
8. Wash the membrane in wash buffer three times for 10 minutes each.
9. Apply the diluted HRP conjugated secondary antibody in blocking buffer (as per manufacturers instructions) and incubate 1 hour at room temperature.
10. Wash the blot in wash buffer three times for 10 minutes each (this step can be repeated as required to reduce background).
11. Apply the detection reagent of choice in accordance with the manufacturers instructions.
Note: Tween-20 can be added to the blocking or antibody dilution buffer at a final concentration of 0.05-0.2%.

*The above information is only intended as a guide. The researcher should determine what protocol best meets their needs.

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for PER2 Antibody - BSA Free

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  • Q: Would you please let me know if there is any updated data available regarding the testing of this antibody on mouse by WB?

    A: We do not have an image showing the detection of NB100-125 in mouse tissues, however we will 100% guarantee this item to work as stated on the datasheet.

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