Pericentrin Antibody - BSA Free

Novus Biologicals | Catalog # NB100-61071

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Cited:

Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Pericentrin Primary Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 175 and 225 of human Pericentrin/Kendrin using the numbering given in entry NP_006022.3 (GeneID 5116).

Marker

Centrosome Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

378 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Pericentrin Antibody - BSA Free

Immunohistochemistry: Pericentrin Antibody [NB100-61071]

Immunohistochemistry: Pericentrin Antibody [NB100-61071]

Pericentrin-Antibody-Immunohistochemistry-NB100-61071-img0016.jpg
Western Blot: Pericentrin Antibody [NB100-61071]

Western Blot: Pericentrin Antibody [NB100-61071]

Western Blot: Pericentrin Antibody [NB100-61071] - Whole cell lysate (10 ug) from HEK293T, HeLa, Hep-G2, and K-562 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Pericentrin-Kendrinantibody used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 second.
Immunocytochemistry/ Immunofluorescence: Pericentrin Antibody [NB100-61071]

Immunocytochemistry/ Immunofluorescence: Pericentrin Antibody [NB100-61071]

Immunocytochemistry/Immunofluorescence: Pericentrin Antibody [NB100-61071] - Formaldehyde-fixed asynchronous HeLa cells. Antibody: Affinity purified rabbit anti-Pericentrin/Kendrin used at a dilution of 1:1,000 (1ug/ml). Detection: Red-fluorescent goat anti-rabbit IgG-heavy and light chain cross-adsorbed Antibody DyLight (R) 594 Conjugated used at a dilution of 1:100. Counterstain. DAPI (blue) and Phalloidin (green)
Immunoprecipitation: Pericentrin Antibody [NB100-61071]

Immunoprecipitation: Pericentrin Antibody [NB100-61071]

Immunoprecipitation: Pericentrin Antibody [NB100-61071] - Detection of human Pericentrin/Kendrin by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Pericentrin/Kendrin antibody NB100-61071 (lot 3) used for IP at 6 ug per reaction. Pericentrin/Kendrin was also immunoprecipitated by a previous lot of this antibody (lot 1). For blotting immunoprecipitated Pericentrin/Kendrin, NB100-61071 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Pericentrin Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: Pericentrin Antibody - BSA Free [NB100-61071] -

Immunofluorescent analysis of cilia in renal tissues. (A) Sections (4 μm) of renal parenchymal tissues and tumor tissues were stained with DAPI, acetylated-alpha -tubulin (Ac-tub) and pericentrin (PCNT) to mark cell nuclei and cilia. Presented images are maximal projections of confocal images of typical parenchymal tissue and a representative ccRCC. Scale bars 20 μm. (B) Normalized cilia frequencies of renal tumors, shown are paired quantifications of n = 20 samples. The plot compares the two cilia quantification methodologies described; data was obtained by immunofluorescent (IF) confocal image acquisition or scoring of immunohistochemical (IHC) stained sections. Statistics were determined by performing a paired t-test at a 95% confidence interval. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/23369289), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for Pericentrin Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:1000 - 1:5000

Immunohistochemistry-Paraffin

1:10 - 1:500

Immunoprecipitation

2 - 10 ug/mg lysate

Western Blot

1:2000 - 1:10000
Application Notes
IHC-P was reported in scientific literature (PMID: 23369289). ICC/IF reactivity reported in scientific literature (PMID: 24316073). For ICC/IF, formaldehyde or acetone fixation is recommended. Permeabilization with Triton X-100 is recommended for formaldehyde-fixed cells.

Reviewed Applications

Read 1 review rated 1 using NB100-61071 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Pericentrin

Pericentrin (PCNT) is a large centrosomal protein that binds calmodulin and mediates assembly of the mitotic spindle apparatus. Depletion of PCNT has been shown to cause apoptosis and activation of the G2/M checkpoint. Recently, mutations in the pericentrin gene have been identified as the cause of primordial dwarfism type II (MOPDII) and Seckel syndrome. Additionally, in cells of Seckel syndrome patients, PCNT has been found to be important to the DNA damage response and ATR-dependent checkpoint signaling.

Alternate Names

Kendrin, KIAA0402, MOPD2, PCN, PCNT2pericentrin B, PCNTB, pericentrin, pericentrin 2 (kendrin), pericentrin, kendrin (KIAA0402)10KENPCTN2, pericentrin-2, pericentrin-380, Pericentrin-B, SCKL4kendrin

Entrez Gene IDs

5116 (Human)

Gene Symbol

PCNT

UniProt

O95613 (Human)

Additional Pericentrin Products

Product Documents for Pericentrin Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Pericentrin Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Pericentrin Antibody - BSA Free

Customer Reviews for Pericentrin Antibody - BSA Free (1)

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Customer Images

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  • Pericentrin Antibody
    Name: Jerrold Turner
    Application: Immunocytochemistry
    Sample Tested: Adult small intestine
    Species: Mouse
    Verified Customer | Posted 08/16/2021
    Smear of 2% paraformaldehyde/lysine/periodate (PLP) fixed mouse small itestinal epithelial cells stained for ZO-1 (green) and pericentrin. No staining is detected.
    We tested the antibody on smears (cytology-like preps) of mouse small intestinal epithelial cells fixed with 2% paraformaldehyde/lysine/periodate (PLP; McLean and Nakane. J Histochem Cytochem, 1974) or-20°C methanol. After permeabilizing with 0.1% saponin (PLP-fixed only) and blocking with 5% donkey serum (in PBS), slides were incubated with antibodies at the dilutions indicated below and a rat monoclonal anti-ZO-1 (tight junction protein) antibody (clone R40.76) for 2 hours at room temperature. After washing, species-specific secondary antibodies (cross-adsorbed) produced in goat were applied.
    Pericentrin Antibody - BSA Free NB100-61071
    Bio-Techne Response
    This review was submitted through the legacy Novus Innovators Program, reflecting a new species or application tested on a primary antibody.

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Protocols

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FAQs for Pericentrin Antibody - BSA Free

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  • Q: I ordered your antibody 3 weeks ago and I received it today. The product is Pericentrin Antibody (NB100-61071) (0.1 mg). I did not have experience for doing immunofluorescence with this antibody before. I note that you used acetone or NBF to fix cell in the manual of your product. But the description is too simple, so could you send me a more detailed protocol about doing immunofluorescence with pericentrin antibody?

    A: Please see the following link for our ICC protocol. I hope this helps!

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