Perilipin Antibody (GT2781) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-13615

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Predicted:

Bovine (94%), Porcine (92%), Rat (92%), Sheep (94%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # GT2781

Format

Azide and BSA Free
View all Perilipin Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human Perilipin. The exact sequence is proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

56 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Centrifuge briefly prior to opening.

Scientific Data Images for Perilipin Antibody (GT2781) - Azide and BSA Free

Immunohistochemistry-Paraffin: Perilipin Antibody (GT2781) [NBP3-13615]

Immunohistochemistry-Paraffin: Perilipin Antibody (GT2781) [NBP3-13615]

Immunohistochemistry-Paraffin: Perilipin Antibody (GT2781) [NBP3-13615] - Perilipin antibody [GT2781] detects Perilipin protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse white adipocyte. Perilipin stained by Perilipin antibody [GT2781] (NBP3-13615) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Western Blot: Perilipin Antibody (GT2781) [NBP3-13615]

Western Blot: Perilipin Antibody (GT2781) [NBP3-13615]

Western Blot: Perilipin Antibody (GT2781) [NBP3-13615] - Mouse tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Perilipin antibody [GT2781] (NBP3-13615) diluted at 1:5000. The HRP-conjugated anti-mouset IgG antibody (NBP2-19382) was used to detect the primary antibody.
Perilipin Antibody (GT2781) - Azide and BSA Free

Western Blot: Perilipin Antibody (GT2781) [NBP3-13615] -

Human tissue extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Perilipin 1 antibody [GT2781] (NBP3-13615) diluted at 1:2000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Perilipin Antibody (GT2781) - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: Perilipin Antibody (GT2781) [NBP3-13615] -

Perilipin 1 antibody [GT2781] detects Perilipin 1 protein at lipid droplet by immunofluorescent analysis.
Sample: Mock and differentiated 3T3-L1 cells were fixed in ice-cold MeOH for 5 min.
Green: Perilipin 1 stained by Perilipin 1 antibody [GT2781] (NBP3-13615) diluted at 1:500.
Blue: Fluoroshield with DAPI.

Applications for Perilipin Antibody (GT2781) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

Assay dependent

Immunohistochemistry

Assay dependent

Immunohistochemistry-Paraffin

Assay dependent

Western Blot

1:1000-1:10000

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS, 20% Glycerol

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Perilipin

The perilipin family includes lipid droplet-associated protein (perilipin) and adipose differentiation-related protein (adipophilin). Perilipin is a modulator of both adipocyte lipid metabolism and adipophilinis involved in the development and maintenance of adipose tissue. Perilipin acts as a protective coating from the body's natural lipases--such as hormone-sensitive lipase--that break triglycerides into glycerol and free fatty acids for use in metabolism, a process called lipolysis. It is also an important regulator of lipid storage and its expression is elevated in obese animals and humans.

Alternate Names

PERI, PLIN, PLIN1

Gene Symbol

PLIN1

Additional Perilipin Products

Product Documents for Perilipin Antibody (GT2781) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for Perilipin Antibody (GT2781) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Diabetes
Obesity

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Protocols

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FAQs

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