Periostin/OSF-2 Antibody (CL12090) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-44324

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # CL12090

Format

Azide and BSA Free
View all Periostin/OSF-2 Primary Antibodies »

Product Specifications

Immunogen

This antibody was generated using a synthetic peptide of Q15063, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Periostin/OSF-2 Antibody (CL12090) - Azide and BSA Free

Periostin/OSF-2 Antibody (CL12090) - Azide and BSA Free Immunohistochemistry-Paraffin: Periostin/OSF-2 Antibody [NBP3-44324]

Immunohistochemistry-Paraffin: Periostin/OSF-2 Antibody [NBP3-44324]

Staining of human breast cancer shows strong positivity in extracellular matrix.
Periostin/OSF-2 Antibody (CL12090) - Azide and BSA Free Immunohistochemistry-Paraffin: Periostin/OSF-2 Antibody [NBP3-44324]

Immunohistochemistry-Paraffin: Periostin/OSF-2 Antibody [NBP3-44324]

Analysis in human fallopian tube and liver tissues using NBP3-44324 antibody. Corresponding Periostin/OSF-2 RNA-seq data are presented for the same tissues.
Periostin/OSF-2 Antibody (CL12090) - Azide and BSA Free Immunohistochemistry-Paraffin: Periostin/OSF-2 Antibody [NBP3-44324]

Immunohistochemistry-Paraffin: Periostin/OSF-2 Antibody [NBP3-44324]

Staining of human colon shows strong positivity in extracellular matrix, as well as moderate positivity in glandular cells.
Periostin/OSF-2 Antibody (CL12090) - Azide and BSA Free Immunohistochemistry-Paraffin: Periostin/OSF-2 Antibody [NBP3-44324]

Immunohistochemistry-Paraffin: Periostin/OSF-2 Antibody [NBP3-44324]

Staining of human fallopian tube shows strong positivity in extracellular matrix.
Periostin/OSF-2 Antibody (CL12090) - Azide and BSA Free Immunohistochemistry-Paraffin: Periostin/OSF-2 Antibody [NBP3-44324]

Immunohistochemistry-Paraffin: Periostin/OSF-2 Antibody [NBP3-44324]

Staining of human glioblastoma shows moderate to strong positivity.
Periostin/OSF-2 Antibody (CL12090) - Azide and BSA Free Immunohistochemistry-Paraffin: Periostin/OSF-2 Antibody [NBP3-44324]

Immunohistochemistry-Paraffin: Periostin/OSF-2 Antibody [NBP3-44324]

Staining of human liver shows no positivity in hepatocytes as expected.
Periostin/OSF-2 Antibody (CL12090) - Azide and BSA Free Immunocytochemistry/ Immunofluorescence: Periostin/OSF-2 Antibody [NBP3-44324]

Immunocytochemistry/ Immunofluorescence: Periostin/OSF-2 Antibody [NBP3-44324]

Staining of U-138 cells using the Anti-Periostin/OSF-2 monoclonal antibody, showing specific staining in vesicles in green. Microtubule- and nuclear probes are visualized in red and blue, respectively.

Applications for Periostin/OSF-2 Antibody (CL12090) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry-Paraffin

1:500 - 1:1000
Application Notes
Immunocytochemistry/ Immunofluorescence/IF Fixation Permeabilization: Use PFA/Triton X-100. For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS with Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Periostin/OSF-2

Periostin is a disulfide linked 90 kDa 811 amno acid protein originally isolated as a osteoblastspecific factor that functions as a cell adhesion molecule for preosteoblasts and is thought to be involved in osteoblast recruitment, attachment and spreading. Additionally, periostin expression has previously been shown to be significantly increased by both transforming growth factor beta-1(TGFbeta1) and bone morphogenetic protein (BMP)-2. OSF-2 has a typical signal sequence, followed by a cysteine-rich domain, a fourfold repeated domain and a C-terminal domain. The fourfold repeated domain of OSF-2 shows homology with the insect protein fasciclin. Periostin mRNA is expressed in the developing mouse embryonic and fetal heart, and that it is localized to the endocardial cushions that ultimately divide the primitive heart tube into a four-chambered heart.

Long Name

Osteoblast Specific Factor 2

Alternate Names

Fasciclin I-like, OSF-2, OSF2, POSTN, TRIF52

Gene Symbol

POSTN

Additional Periostin/OSF-2 Products

Product Documents for Periostin/OSF-2 Antibody (CL12090) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for Periostin/OSF-2 Antibody (CL12090) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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