Periostin/OSF-2 Antibody (OTI1A1)

Novus Biologicals | Catalog # NBP1-47922

Novus Biologicals

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Immunocytochemistry/ Immunofluorescence, IF/IHC

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgM Clone # OTI1A1
View all Periostin/OSF-2 Primary Antibodies »

Product Specifications

Immunogen

Human recombinant protein fragment corresponding to amino acids 1-230 of human Periostin/OSF-2 (NP_006466) produced in E.coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Specificity

This antibody will possibly detect all isoforms encoding the amino acid regions 1-230 of human Periostin/OSF-2.

Clonality

Monoclonal

Host

Mouse

Isotype

IgM

Theoretical MW

93.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Periostin/OSF-2 Antibody (OTI1A1)

Western Blot: Periostin/OSF-2 Antibody (OTI1A1) [NBP1-47922]

Western Blot: Periostin/OSF-2 Antibody (OTI1A1) [NBP1-47922]

Western Blot: Periostin/OSF-2 Antibody (1A1) [NBP1-47922] - Periostin antibody ( 47 ) at 1:500 dilution + lysate from HeLa cells transfected with human Periostin gene expression vector.
Immunocytochemistry/ Immunofluorescence: Periostin/OSF-2 Antibody (OTI1A1) [NBP1-47922]

Immunocytochemistry/ Immunofluorescence: Periostin/OSF-2 Antibody (OTI1A1) [NBP1-47922]

Immunocytochemistry/Immunofluorescence: Periostin/OSF-2 Antibody (1A1) [NBP1-47922] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY POSTN.

Applications for Periostin/OSF-2 Antibody (OTI1A1)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

Reactivity reported in (PMID:22512703).

Western Blot

1:500

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Periostin/OSF-2

Periostin is a disulfide linked 90 kDa, 811 amino acid protein originally isolated as a osteoblast-specific factor that functions as a cell adhesion molecule for preosteoblasts and is thought to be involved in osteoblast recruitment, attachment and spreading. Additionally, periostin expression has previously been shown to be significantly increased by both transforming growth factor beta 1(TGF beta 1) and bone morphogenetic protein (BMP2). Periostin mRNA is expressed in the developing mouse embryonic and fetal heart, and that it is localized to the endocardial cushions that ultimately divide the primitive heart tube into a four-chambered heart. Abnormal expression of periostin is also linked to angiogenesis and metastatsis in epithelial tumors

Long Name

Osteoblast Specific Factor 2

Alternate Names

Fasciclin I-like, OSF-2, OSF2, POSTN, TRIF52

Entrez Gene IDs

10631 (Human)

Gene Symbol

POSTN

UniProt

Q15063

Additional Periostin/OSF-2 Products

Product Documents for Periostin/OSF-2 Antibody (OTI1A1)

Certificate of Analysis

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Product Specific Notices for Periostin/OSF-2 Antibody (OTI1A1)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Periostin/OSF-2 Antibody (OTI1A1)

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Protocols

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