Peripheral Node Addressin Antibody (MECA-79R) - BSA Free

Detection of Peripheral Node Addressin in Human Tonsil via seqIF™ staining on COMET™

Peripheral Node Addressin was detected in immersion fixed paraffin-embedded sections of human Tonsil using Rat Anti-Human Peripheral Node Addressin (MECA-79R), Monoclonal Antibody (Catalog #NBP2-78792) at 1:50 dilution at 37° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH). Tissue was stained using the Alexa Fluor™ 647 Goat anti-Rat IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647RT) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to endothelial cells. Protocol available in COMET™ Panel Builder.

Western Blot: Peripheral Node Addressin Antibody (MECA-79R)BSA Free [NBP2-78792]

Western Blot: Peripheral Node Addressin Antibody (MECA-79R) [NBP2-78792] - Total protein from human Tonsil, Lymph node, Spleen and mouse Spleen was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-PNAd in blocking buffer and detected with an anti-rat HRP secondary antibody using West Pico PLUS chemiluminescence detection reagent.

Immunohistochemistry-Paraffin: Peripheral Node Addressin Antibody (MECA-79R) - BSA Free [NBP2-78792]

Immunohistochemistry-Paraffin: Peripheral Node Addressin Antibody (MECA-79R) [NBP2-78792] - Analysis of FFPE mouse adipose tissue section (with lymph node areas) using Peripheral Node Addressin antibody (clone MECA-79) at 1:100. The staining was developed with HRP-DAB detection method and the counterstaining was performed using hematoxylin. This Peripheral Node Addressin antibody generated a strong and specific staining of MECA-79 antigen in the the cytoplasm and the membranes of high endothelial venules (HEVs) aka peripheral lymph node addressin (PNAd) in lymph node areas of tested adipose tissue section.