Peroxiredoxin 1 Antibody

Novus Biologicals | Catalog # NBP1-30973

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Bovine (95%), Canine (97%), Rhesus Macaque (98%). Backed by our 100% Guarantee.

Applications

Knockout Validated, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all Peroxiredoxin 1 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human Peroxiredoxin 1. The exact sequence is proprietary.

Reactivity Notes

Chicken (86%), Zebrafish (80%).

Localization

Cytoplasm, Melanosome

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

22 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Peroxiredoxin 1 Antibody

Western Blot: Peroxiredoxin 1 Antibody [NBP1-30973]

Western Blot: Peroxiredoxin 1 Antibody [NBP1-30973]

Western Blot: Peroxiredoxin 1 Antibody [NBP1-30973] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with PRX I antibody [N1C2] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunocytochemistry/ Immunofluorescence: Peroxiredoxin 1 Antibody [NBP1-30973]

Immunocytochemistry/ Immunofluorescence: Peroxiredoxin 1 Antibody [NBP1-30973]

Immunocytochemistry/Immunofluorescence: Peroxiredoxin 1 Antibody [NBP1-30973] - HeLa cells were fixed in ice-cold MeOH for 5 min. Green: PRX I stained by PRX I antibody [N1C2] diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.Blue: Fluoroshield with DAPI.
Immunohistochemistry-Paraffin: Peroxiredoxin 1 Antibody [NBP1-30973]

Immunohistochemistry-Paraffin: Peroxiredoxin 1 Antibody [NBP1-30973]

Immunohistochemistry-Paraffin: Peroxiredoxin 1 Antibody [NBP1-30973] - Mouse lung. PRX I antibody [N1C2] dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
Western Blot: Peroxiredoxin 1 Antibody [NBP1-30973]

Western Blot: Peroxiredoxin 1 Antibody [NBP1-30973]

Western Blot: Peroxiredoxin 1 Antibody [NBP1-30973] - Sample (30 ug of whole cell lysate) A:NIH-3T3 12% SDS PAGE; antibody diluted at 1:1000.
Western Blot: Peroxiredoxin 1 Antibody [NBP1-30973]

Western Blot: Peroxiredoxin 1 Antibody [NBP1-30973]

Western Blot: Peroxiredoxin 1 Antibody [NBP1-30973] - Sample (30 ug of whole cell lysate) A: 293T 12% SDS PAGE; antibody diluted at 1:1000.
Western Blot: Peroxiredoxin 1 Antibody [NBP1-30973]

Western Blot: Peroxiredoxin 1 Antibody [NBP1-30973]

Western Blot: Peroxiredoxin 1 Antibody [NBP1-30973] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with PRX I antibody [N1C2] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Applications for Peroxiredoxin 1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Peroxiredoxin 1

This gene encodes a member of the peroxiredoxin family of antioxidant enzymes, which reduce hydrogen peroxide and alkyl hydroperoxides. The encoded protein may play an antioxidant protective role in cells, and may contribute to the antiviral activity of CD8(+) T-cells. This protein may have a proliferative effect and play a role in cancer development or progression. Three transcript variants encoding the same protein have been identified for this gene.

Alternate Names

NKEF-A, PRDX1, PRX1, TDPX2

Gene Symbol

PRDX1

Additional Peroxiredoxin 1 Products

Product Documents for Peroxiredoxin 1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Peroxiredoxin 1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Protocols

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