Peroxiredoxin 3 Antibody (1E3-B2) - Azide and BSA Free

Novus Biologicals | Catalog # H00010935-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 1E3-B2

Format

Azide and BSA Free
View all Peroxiredoxin 3 Primary Antibodies »

Product Specifications

Immunogen

PRDX3 (AAH02685, 1 a.a. ~ 256 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MAAAVGRLLRASVARHVSAIPWGISATAALRPAACGRTSLTNLLCSGSSQAKLFSTSSSCHAPAVTQHAPYFKGTAVVNGEFKDLSLDDFKGKYLVLFFYPLDFTFVCPTEIVAFSDKANEFHDVNCEVVAVSVDSHFSHLAWINTPRKNGGLGHMNIALLSDLTKQISRDYGVLLEGSGLALRGLFIIDPNGVIKHLSVNDLPVGRSVEETLRLVKAFQYVETHGEVCPANWTPDSPTIKPSPAASKEYFQKVNQ

Specificity

PRDX3 - peroxiredoxin 3

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for Peroxiredoxin 3 Antibody (1E3-B2) - Azide and BSA Free

Western Blot: Peroxiredoxin 3 Antibody (1E3-B2) [H00010935-M01]

Western Blot: Peroxiredoxin 3 Antibody (1E3-B2) [H00010935-M01]

Western Blot: Peroxiredoxin 3 Antibody (1E3-B2) [H00010935-M01] - Analysis of PRDX3 expression in transfected 293T cell line by PRDX3 monoclonal antibody (M01), clone 1E3-B2.Lane 1: PRDX3 transfected lysate (Predicted MW: 27.7 KDa).Lane 2: Non-transfected lysate.
Immunocytochemistry/ Immunofluorescence: Peroxiredoxin 3 Antibody (1E3-B2) [H00010935-M01]

Immunocytochemistry/ Immunofluorescence: Peroxiredoxin 3 Antibody (1E3-B2) [H00010935-M01]

Immunocytochemistry/Immunofluorescence: Peroxiredoxin 3 Antibody (1E3-B2) [H00010935-M01] - Analysis of monoclonal antibody to PRDX3 on HeLa cell. Antibody concentration 10 ug/ml.
Immunoprecipitation: Peroxiredoxin 3 Antibody (1E3-B2) [H00010935-M01]

Immunoprecipitation: Peroxiredoxin 3 Antibody (1E3-B2) [H00010935-M01]

Immunoprecipitation: Peroxiredoxin 3 Antibody (1E3-B2) [H00010935-M01] - Analysis of PRDX3 transfected lysate using anti-PRDX3 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PRDX3 MaxPab rabbit polyclonal antibody.
ELISA: Peroxiredoxin 3 Antibody (1E3-B2) [H00010935-M01]

ELISA: Peroxiredoxin 3 Antibody (1E3-B2) [H00010935-M01]

ELISA: Peroxiredoxin 3 Antibody (1E3-B2) [H00010935-M01] - Detection limit for recombinant GST tagged PRDX3 is approximately 1ng/ml as a capture antibody.

Applications for Peroxiredoxin 3 Antibody (1E3-B2) - Azide and BSA Free

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactive against recombinant protein with GST tag on ELISA and immunofluorescence. GST tag alone is used as a negative control.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Peroxiredoxin 3

This gene encodes a protein with antioxidant function and is localized in the mitochondrion. This gene shows significant nucleotide sequence similarity to the gene coding for the C22 subunit of Salmonella typhimurium alkylhydroperoxide reductase. Expression of this gene product in E. coli deficient in the C22-subunit gene rescued resistance of the bacteria to alkylhydroperoxide. The human and mouse genes are highly conserved, and they map to the regions syntenic between mouse and human chromosomes. Sequence comparisons with recently cloned mammalian homologues suggest that these genes consist of a family that is responsible for regulation of cellular proliferation, differentiation, and antioxidant functions. Two transcript variants encoding two different isoforms have been found for this gene.

Alternate Names

AOP-1, MER5, PRDX3, SP-22

Entrez Gene IDs

10935 (Human)

Gene Symbol

PRDX3

OMIM

604769 (Human)

UniProt

AAH02685 (Human)

Additional Peroxiredoxin 3 Products

Product Documents for Peroxiredoxin 3 Antibody (1E3-B2) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for Peroxiredoxin 3 Antibody (1E3-B2) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Oxidative Stress
Redox Enzymes

Citations for Peroxiredoxin 3 Antibody (1E3-B2) - Azide and BSA Free

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Protocols

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FAQs

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