Peroxiredoxin 3 Antibody (1F8) - BSA Free

Novus Biologicals | Catalog # NBP2-59425

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG3 Kappa Clone # 1F8

Format

BSA Free
View all Peroxiredoxin 3 Primary Antibodies »

Product Specifications

Immunogen

Recombinant human Peroxiredoxin3 (63-256aa) purified from E. coli

Clonality

Monoclonal

Host

Mouse

Isotype

IgG3 Kappa

Scientific Data Images for Peroxiredoxin 3 Antibody (1F8) - BSA Free

Western Blot: Peroxiredoxin 3 Antibody (1F8)BSA Free [NBP2-59425]

Western Blot: Peroxiredoxin 3 Antibody (1F8)BSA Free [NBP2-59425]

Western Blot: Peroxiredoxin 3 Antibody (1F8) [NBP2-59425] - Lane 1: Recombinant protein, Lane 2: HeLa cell lysates, Lane 3: HepG2 cell lysates, Lane 4: TF1 cell lysates, Lane 5: U87MG cell lysates, Lane 6: Raji cell lysates, Lane 7: 293T cell lysates, Lane 8: Jurkat cell lysates, Lane 9: MCF7 cell lysates
Immunocytochemistry/ Immunofluorescence: Peroxiredoxin 3 Antibody (1F8) - BSA Free [NBP2-59425]

Immunocytochemistry/ Immunofluorescence: Peroxiredoxin 3 Antibody (1F8) - BSA Free [NBP2-59425]

Immunocytochemistry/Immunofluorescence: Peroxiredoxin 3 Antibody (1F8) [NBP2-59425] - Analysis of Peroxiredoxin3 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human Peroxiredoxin3 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).

Applications for Peroxiredoxin 3 Antibody (1F8) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.4), 10% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Peroxiredoxin 3

The peroxiredoxin (PRX) family comprises six antioxidant proteins, PRX I, II, III, IV, V and VI, which protect cells from reactive oxygen species (ROS) by preventing the metal-catalyzed oxidation of enzymes. The PRX proteins primarily utilize thioredoxin as the electron donor for antioxidation, although they are fairly promiscuous with regard to the hydroperoxide substrate. In addition to protection from ROS, peroxiredoxins are also involved in cell proliferation, differentiation and gene expression. PRX I, II, IV and VI show diffuse cytoplasmic localization, while PRX III and V exhibit distinct mitochondrial localization. The human PRX I gene encodes a protein that is expressed in several tissues, including liver, kidney, testis, lung and nervous system. PRX II is expressed in testis, while PRX III shows expression in lung. PRX I, II and III are overexpressed in breast cancer and may be involved in its development or progression. Upregulated protein levels of PRX I and II in Alzheimer's disease (AD) and Down syndrome (DS) indicate the involvement of PRX I and II in their pathogenesis.

Alternate Names

AOP-1, MER5, PRDX3, SP-22

Gene Symbol

PRDX3

Additional Peroxiredoxin 3 Products

Product Documents for Peroxiredoxin 3 Antibody (1F8) - BSA Free

Certificate of Analysis

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Product Specific Notices for Peroxiredoxin 3 Antibody (1F8) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Oxidative Stress
Redox Enzymes

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Protocols

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