Peroxiredoxin 4 Antibody (SAIC-40A-24) - Azide and BSA Free

Name: Peroxiredoxin 4 Antibody (SAIC-40A-24) - Azide and BSA Free
Brand: Novus Biologicals
SKU: NBP3-20105
Price: 199 USD
Availability: InStock

Novus Biologicals | Catalog # NBP3-20105

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Product Documents

Recombinant Monoclonal Antibody

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Product Specifications
Scientific Data
Applications
Flow Cytometry
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Mass Spectrometry

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Kappa Clone # SAIC-40A-24

Format

Azide and BSA Free

View all Peroxiredoxin 4 Primary Antibodies »

Product Specifications

Immunogen

Peptide "QITLNDLPVGR", derived from the cell redox-regulator Peroxiredoxin-4, conjugated to KLH.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG Kappa

Scientific Data Images for Peroxiredoxin 4 Antibody (SAIC-40A-24) - Azide and BSA Free

Western Blot: Peroxiredoxin 4 Antibody (SAIC-40A-24) - Azide and BSA Free [NBP3-20105] -

Western Blot: Peroxiredoxin 4 Antibody (SAIC-40A-24) - Azide and BSA Free [NBP3-20105] - Human liver(A) and human pancreas(B) tissue lysates (35ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of SAIC-40A-24 (NBP3-20105) at 0.001 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.

Immunocytochemistry/Immunofluorescence: Peroxiredoxin 4 Antibody (SAIC-40A-24) - Azide and BSA Free [NBP3-20105] -

Immunocytochemistry/Immunofluorescence: Peroxiredoxin 4 Antibody (SAIC-40A-24) - Azide and BSA Free [NBP3-20105] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells permeabilized with 0.15% Triton stained with the chimeric rabbit IgG version of SAIC-40A-24 (NBP3-20105) (1:100 dilution) for 1h followed by Alexa Fluor(R) 488 secondary antibody (1:1000 dilution), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-20105, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.

Flow Cytometry: Peroxiredoxin 4 Antibody (SAIC-40A-24) - Azide and BSA Free [NBP3-20105] -

Flow Cytometry: Peroxiredoxin 4 Antibody (SAIC-40A-24) - Azide and BSA Free [NBP3-20105] - Paraformaldehyde fixed HeLa cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (isotype control, black line) or the rabbit IgG version of SAIC-40A-24 (NBP3-20105, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor(R) 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.