Pescadillo Antibody

Novus Biologicals | Catalog # NB110-40548

Novus Biologicals
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Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation

Cited:

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all Pescadillo Primary Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 535 and the C-terminus (residue 588) of human Pescadillo using the numbering given in entry NP_055118.1 (GeneID 23481).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Pescadillo Antibody

Western Blot: Pescadillo Antibody [NB110-40548]

Western Blot: Pescadillo Antibody [NB110-40548]

Western Blot: Pescadillo Antibody [NB110-40548] - Detection of human Pescadillo by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Pescadillo antibody NB110-40548 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Immunohistochemistry: Pescadillo Antibody [NB110-40548]

Immunohistochemistry: Pescadillo Antibody [NB110-40548]

Immunohistochemistry: Pescadillo Antibody [NB110-40548] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti- Pescadillo used at a dilution of 1:200 (1ug/ml). Detection: DAB
Western Blot: Pescadillo Antibody [NB110-40548]

Western Blot: Pescadillo Antibody [NB110-40548]

Western Blot: Pescadillo Antibody [NB110-40548] - Detection of Human Pescadillo on HeLa whole cell lysate using NB110-40548. Pescadillo was also immunoprecipitated by another rabbit anti-Pescadillo antibody and NB110-40548.
Immunohistochemistry-Paraffin: Pescadillo Antibody [NB110-40548]

Immunohistochemistry-Paraffin: Pescadillo Antibody [NB110-40548]

Immunohistochemistry-Paraffin: Pescadillo Antibody [NB110-40548] - FFPE section of human breast adenocarcinoma. Affinity purified rabbit anti-Pescadillo used at a dilution of 1:250.
Immunoprecipitation: Pescadillo Antibody [NB110-40548]

Immunoprecipitation: Pescadillo Antibody [NB110-40548]

Immunoprecipitation: Pescadillo Antibody [NB110-40548] - Detection of human Pescadillo by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Pescadillo antibody NB110-40548 used for IP at 3 ug per reaction. Pescadillo was also immunoprecipitated by another rabbit anti-Pescadillo antibody. For blotting immunoprecipitated Pescadillo, NB110-40548 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.

Applications for Pescadillo Antibody

Application
Recommended Usage

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:100-1:500

Immunoprecipitation

2-5 mcg/mg

Western Blot

1:2000-1:10000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Pescadillo

This gene encodes a protein that is abnormally elevated in malignant tumors of astrocytic origin. It is a strongly conserved gene containing a BRCT domain that is essential for the activity of this gene product. The gene plays a crucial role in cell proliferation and may be necessary for oncogenic transformation and tumor progression.

Alternate Names

pescadillo homolog, pescadillo homolog 1, containing BRCT domain (zebrafish), PESpescadillo (zebrafish) homolog 1, containing BRCT domain

Entrez Gene IDs

23481 (Human)

Gene Symbol

PES1

UniProt

NP_055118.1 (Human)

Additional Pescadillo Products

Product Documents for Pescadillo Antibody

Certificate of Analysis

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Product Specific Notices for Pescadillo Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Pescadillo Antibody

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Protocols

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