PEX19 Antibody (554) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-43544

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Predicted:

Bovine (95%), Rat (93%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 554

Format

Azide and BSA Free
View all PEX19 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human PEX19. The exact sequence is proprietary.

Reactivity Notes

Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

33 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PEX19 Antibody (554) - Azide and BSA Free

Western Blot: PEX19 Antibody (554) [NBP2-43544]

Western Blot: PEX19 Antibody (554) [NBP2-43544]

Western Blot: PEX19 Antibody (554) [NBP2-43544] - Analysis of A. 50 ug mouse liver lysate/extract 10 % SDS-PAGE PEX19 antibody [554] dilution: 1:1000.
Immunohistochemistry-Paraffin: PEX19 Antibody (554) [NBP2-43544]

Immunohistochemistry-Paraffin: PEX19 Antibody (554) [NBP2-43544]

Immunohistochemistry-Paraffin: PEX19 Antibody (554) [NBP2-43544] - Human colon carcinoma. PEX19 antibody [554] dilution: 1:200. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Flow Cytometry: PEX19 Antibody (554) [NBP2-43544]

Flow Cytometry: PEX19 Antibody (554) [NBP2-43544]

Flow Cytometry: PEX19 Antibody (554) [NBP2-43544] - THP-1 cell. Black: Unlabelled sample was used as a control. Red: PEX19 antibody [554] dilution: 1:50. Acquisition of 20,000 events were collected using a Dylight 488-conjugated secondary antibody for FACS analysis.
Western Blot: PEX19 Antibody (554) [NBP2-43544]

Western Blot: PEX19 Antibody (554) [NBP2-43544]

Western Blot: PEX19 Antibody (554) [NBP2-43544] - Analysis of 30 ug whole cell lysate. A: Jurkat B: Raji C: K562 D: THP-1 E: HL-60 F: NCI-H929 10% SDS PAGE diluted at 1:5000.
PEX19 Antibody (554) - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: PEX19 Antibody (554) [NBP2-43544] -

PEX19 antibody [GT554] detects PEX19 protein at cytoplasm by immunofluorescent analysis.
Sample: Jurkat cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: PEX19 stained by PEX19 antibody [GT554] (NBP2-43544) diluted at 1:500.
Blue: Fluoroshield with DAPI.

Applications for PEX19 Antibody (554) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:50-1:200

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:10000

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: PEX19

PEX19 is necessary for early peroxisomal biogenesis. It acts both as a cytosolic chaperone and as an import receptor for peroxisomal membrane proteins (PMPs). Peroxins (PEXs) are proteins that are essential for the assembly of functional peroxisomes. The peroxisome biogenesis disorders (PBDs) are a group of genetically heterogeneous autosomal recessive, lethal diseases characterized by multiple defects in peroxisome function. The peroxisomal biogenesis disorders are a heterogeneous group with at least 14 complementation groups and with more than 1 phenotype being observed in cases falling into particular complementation groups. Although the clinical features of PBD patients vary, cells from all PBD patients exhibit a defect in the import of one or more classes of peroxisomal matrix proteins into the organelle. Defects in this gene are a cause Zellweger syndrome (ZWS). [provided by RefSeq]

Alternate Names

D1S2223Ehousekeeping gene, 33kD, HK3333 kDa housekeeping protein, Peroxin-19, peroxisomal biogenesis factor 19, Peroxisomal farnesylated proteinFLJ55296, PMP1, PMPI, PXFperoxin-19, PXMP1

Gene Symbol

PEX19

Additional PEX19 Products

Product Documents for PEX19 Antibody (554) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for PEX19 Antibody (554) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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