PFKFB4 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-32538

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Bovine (99%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
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Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human PFKFB4. The exact sequence is proprietary.

Reactivity Notes

Xenopus laevis (85%), Chicken (86%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

54 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PFKFB4 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: PFKFB4 Antibody [NBP1-32538]

Immunocytochemistry/ Immunofluorescence: PFKFB4 Antibody [NBP1-32538]

Immunocytochemistry/Immunofluorescence: PFKFB4 Antibody [NBP1-32538] - Sample: NT2D1 cells.Green: PFKFB4 protein stained by PFKFB4 antibody (NBP1-32538) diluted at 1:500.Blue: Hoechst 33342 staining.
Immunohistochemistry-Paraffin: PFKFB4 Antibody [NBP1-32538]

Immunohistochemistry-Paraffin: PFKFB4 Antibody [NBP1-32538]

Immunohistochemistry-Paraffin: PFKFB4 Antibody [NBP1-32538] - Paraffin-embedded mouse spleen. PFKFB4 antibody diluted at 1:500.
Immunohistochemistry-Paraffin: PFKFB4 Antibody [NBP1-32538]

Immunohistochemistry-Paraffin: PFKFB4 Antibody [NBP1-32538]

Immunohistochemistry-Paraffin: PFKFB4 Antibody [NBP1-32538] - Paraffin-embedded NCI-N87 xenograft, using antibody at 1:500 dilution.
Immunohistochemistry-Paraffin: PFKFB4 Antibody [NBP1-32538]

Immunohistochemistry-Paraffin: PFKFB4 Antibody [NBP1-32538]

Immunohistochemistry-Paraffin: PFKFB4 Antibody [NBP1-32538] - Paraffin-embedded mouse testis. PFKFB4 antibody diluted at 1:500.
PFKFB4 Antibody

Western Blot: PFKFB4 Antibody [NBP1-32538] -

Western Blot: PFKFB4 Antibody [NBP1-32538] - PFKFB4 antibody detects PFKFB4 protein by Western blot analysis. Mouse tissue extracts (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with PFKFB4 antibody (NBP1-32538) diluted at 1:2000.
PFKFB4 Antibody - BSA Free

Western Blot: PFKFB4 Antibody [NBP1-32538] -

Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PFKFB4 antibody (NBP1-32538) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
PFKFB4 Antibody - BSA Free

Western Blot: PFKFB4 Antibody [NBP1-32538] -

Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PFKFB4 antibody (NBP1-32538) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Applications for PFKFB4 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 20% Glycerol

Format

BSA Free

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: PFKFB4

Synthesis and degradation of fructose 2,6-bisphosphate

Long Name

6-Phosphofructo-2-Kinase/ Fructose-2,6-Bisphosphatase 4

Alternate Names

6PF-2-K/Fru-2,6-P2ase 4, PFK/FBPase 4

Gene Symbol

PFKFB4

Additional PFKFB4 Products

Product Documents for PFKFB4 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for PFKFB4 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Protocols

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