PGAM2 Antibody (OTI4E9)

Novus Biologicals | Catalog # NBP2-01142

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat, Monkey, Primate

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # OTI4E9
View all PGAM2 Primary Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human PGAM2(NP_000028) produced in HEK293 cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

28.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PGAM2 Antibody (OTI4E9)

Western Blot: PGAM2 Antibody (4E9) [NBP2-01142] Analysis of extracts (35ug) from 9 different cell lines by using anti-PGAM2 monoclonal antibody.
Immunocytochemistry/ Immunofluorescence: PGAM2 Antibody (OTI4E9) [NBP2-01142]

Immunocytochemistry/ Immunofluorescence: PGAM2 Antibody (OTI4E9) [NBP2-01142]

Immunocytochemistry/Immunofluorescence: PGAM2 Antibody (4E9) [NBP2-01142] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY PGAM2.
Immunohistochemistry-Paraffin: PGAM2 Antibody (OTI4E9) [NBP2-01142]

Immunohistochemistry-Paraffin: PGAM2 Antibody (OTI4E9) [NBP2-01142]

Immunohistochemistry-Paraffin: PGAM2 Antibody (4E9) [NBP2-01142] - Staining of paraffin-embedded Human liver tissue using anti-PGAM2 mouse monoclonal antibody.
Flow Cytometry: PGAM2 Antibody (OTI4E9) [NBP2-01142]

Flow Cytometry: PGAM2 Antibody (OTI4E9) [NBP2-01142]

Flow Cytometry: PGAM2 Antibody (4E9) [NBP2-01142] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PGAM2 antibody, and then analyzed by flow cytometry.
Western Blot: PGAM2 Antibody (OTI4E9) [NBP2-01142]

Western Blot: PGAM2 Antibody (OTI4E9) [NBP2-01142]

Western Blot: PGAM2 Antibody (4E9) [NBP2-01142] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PGAM2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PGAM2.
Immunocytochemistry/ Immunofluorescence: PGAM2 Antibody (OTI4E9) [NBP2-01142]

Immunocytochemistry/ Immunofluorescence: PGAM2 Antibody (OTI4E9) [NBP2-01142]

Immunocytochemistry/Immunofluorescence: PGAM2 Antibody (4E9) [NBP2-01142] - Immunofluorescent staining of HeLa cells using anti-PGAM2 mouse monoclonal antibody.

Applications for PGAM2 Antibody (OTI4E9)

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:50-100

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Western Blot

1:500-2000

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PGAM2

Phosphoglycerate mutase (PGAM) catalyzes the reversible reaction of 3-phosphoglycerate (3-PGA) to 2-phosphoglycerate (2-PGA) in the glycolytic pathway. The PGAM is a dimeric enzyme containing, in different tissues, different proportions of a slow-migrating muscle (MM) isozyme, a fast-migrating brain (BB) isozyme, and a hybrid form (MB). This gene encodes muscle-specific PGAM subunit. Mutations in this gene cause muscle phosphoglycerate mutase eficiency, also known as glycogen storage disease X. [provided by RefSeq].

Alternate Names

BPG-dependent PGAM 2, EC 3.1.3.13, EC 5.4.2.1, EC 5.4.2.4, Muscle-specific phosphoglycerate mutase, PGAM-MGSD10, PGAMMMGC88743, phosphoglycerate mutase 2, phosphoglycerate mutase 2 (muscle), Phosphoglycerate mutase isozyme M

Entrez Gene IDs

5224 (Human)

Gene Symbol

PGAM2

Additional PGAM2 Products

Product Documents for PGAM2 Antibody (OTI4E9)

Certificate of Analysis

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Product Specific Notices for PGAM2 Antibody (OTI4E9)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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