PGAM5 Antibody (CL0624) - BSA Free

Novus Biologicals | Catalog # NBP2-52947

Novus Biologicals
100% Guarantee

Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human

Predicted:

Mouse (94%), Rat (92%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL0624

Format

BSA Free
View all PGAM5 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed using a recombinant protein derived from Q96HS1, with the exact immunogen sequence remaining proprietary.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for PGAM5 Antibody (CL0624) - BSA Free

Western Blot: PGAM5 Antibody (CL0624) [NBP2-52947]

Western Blot: PGAM5 Antibody (CL0624) [NBP2-52947]

Western Blot: PGAM5 Antibody (CL0624) [NBP2-52947] - Lane 1: Marker [kDa] Lane 2: Human cell line RT-4
Immunohistochemistry-Paraffin: PGAM5 Antibody (CL0624) [NBP2-52947]

Immunohistochemistry-Paraffin: PGAM5 Antibody (CL0624) [NBP2-52947]

Immunohistochemistry-Paraffin: PGAM5 Antibody (CL0624) [NBP2-52947] - Staining of human uterus shows moderate cytoplasmic positivity in glandular cells.
Western Blot: PGAM5 Antibody (CL0624) [NBP2-52947]

Western Blot: PGAM5 Antibody (CL0624) [NBP2-52947]

Western Blot: PGAM5 Antibody (CL0624) [NBP2-52947] - Analysis in RT-4 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-PGAM5 antibody. Remaining relative intensity is presented. Loading control: Anti-PARP1.
Immunohistochemistry-Paraffin: PGAM5 Antibody (CL0624) [NBP2-52947]

Immunohistochemistry-Paraffin: PGAM5 Antibody (CL0624) [NBP2-52947]

Immunohistochemistry-Paraffin: PGAM5 Antibody (CL0624) [NBP2-52947] - Staining of human kidney shows either strong or moderate cytoplasmic immunoreactivity in subsets of renal tubules.
Immunohistochemistry-Paraffin: PGAM5 Antibody (CL0624) [NBP2-52947]

Immunohistochemistry-Paraffin: PGAM5 Antibody (CL0624) [NBP2-52947]

Immunohistochemistry-Paraffin: PGAM5 Antibody (CL0624) [NBP2-52947] - Staining of human liver shows moderate cytoplasmic immunoreactivity in hepatocytes.
Immunohistochemistry-Paraffin: PGAM5 Antibody (CL0624) [NBP2-52947]

Immunohistochemistry-Paraffin: PGAM5 Antibody (CL0624) [NBP2-52947]

Immunohistochemistry-Paraffin: PGAM5 Antibody (CL0624) [NBP2-52947] - Staining of human small intestine shows moderate cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: PGAM5 Antibody (CL0624) [NBP2-52947]

Immunohistochemistry-Paraffin: PGAM5 Antibody (CL0624) [NBP2-52947]

Immunohistochemistry-Paraffin: PGAM5 Antibody (CL0624) [NBP2-52947] - Staining of human testis shows moderate to strong cytoplasmic positivity in the seminiferous ducts.
Immunohistochemistry-Paraffin: PGAM5 Antibody (CL0624) [NBP2-52947]

Immunohistochemistry-Paraffin: PGAM5 Antibody (CL0624) [NBP2-52947]

Immunohistochemistry-Paraffin: PGAM5 Antibody (CL0624) [NBP2-52947] - Staining of human tonsil shows strong immunoreactivity in germinal center cells.

Applications for PGAM5 Antibody (CL0624) - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PGAM5

Displays phosphatase activity for serine/threonine residues, and, dephosphorylates and activates MAP3K5kinase. Has apparently no phosphoglycerate mutase activity. May be regulator of mitochondrial dynamics. Substrate fora Keap1-dependent ubiquitin ligase complex. Contributes to the repression of NFE2L2-dependent gene expression

Alternate Names

Bcl-XL-binding protein v68, BXLBv68, EC 3.1.3.16, MGC5352, phosphoglycerate mutase family member 5BXLBV68, serine/threonine-protein phosphatase PGAM5, mitochondrial

Gene Symbol

PGAM5

Additional PGAM5 Products

Product Documents for PGAM5 Antibody (CL0624) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for PGAM5 Antibody (CL0624) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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