Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit

Format

BSA Free
View all PGAM5 Primary Antibodies »

Product Specifications

Immunogen

PGAM5 antibody was raised against a peptide corresponding to 14 amino acids near the carboxyl terminus of human PGAM5. The immunogen is located within the last 50 amino acids of PGAM5.

Clonality

Polyclonal

Host

Rabbit

Scientific Data Images for PGAM5 Antibody - BSA Free

PGAM5 Antibody

Western Blot: PGAM5 Antibody [NBP3-27905] -

Western Blot: PGAM5 Antibody [NBP3-27905] - Antibodies: PGAM5, 0.5 u/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.
PGAM5 Antibody

Immunocytochemistry/ Immunofluorescence: PGAM5 Antibody [NBP3-27905] -

Immunocytochemistry/ Immunofluorescence: PGAM5 Antibody [NBP3-27905] - Immunofluorescent analysis of 4% paraformaldehyde-fixed rat testis labeling PGAM5 at 20 u/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
PGAM5 Antibody

Immunocytochemistry/ Immunofluorescence: PGAM5 Antibody [NBP3-27905] -

Immunocytochemistry/ Immunofluorescence: PGAM5 Antibody [NBP3-27905] - Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse testis labeling PGAM5 with at 10 u/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
PGAM5 Antibody

Western Blot: PGAM5 Antibody [NBP3-27905] -

Western Blot: PGAM5 Antibody [NBP3-27905] - Loading: 15 u of lysates per lane. Antibodies: PGAM5, 0.5 u/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
PGAM5 Antibody

Western Blot: PGAM5 Antibody [NBP3-27905] -

Western Blot: PGAM5 Antibody [NBP3-27905] - Loading: 15 u of lysates per lane. Antibodies: PGAM5, 0.5 u/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
PGAM5 Antibody

Western Blot: PGAM5 Antibody [NBP3-27905] -

Western Blot: PGAM5 Antibody [NBP3-27905] - Antibodies: PGAM5, 0.5 u/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Applications for PGAM5 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

10 - 20 ug/mL

Western Blot

0.5 ug/mL

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PGAM5

Displays phosphatase activity for serine/threonine residues, and, dephosphorylates and activates MAP3K5kinase. Has apparently no phosphoglycerate mutase activity. May be regulator of mitochondrial dynamics. Substrate fora Keap1-dependent ubiquitin ligase complex. Contributes to the repression of NFE2L2-dependent gene expression

Alternate Names

Bcl-XL-binding protein v68, BXLBv68, EC 3.1.3.16, MGC5352, phosphoglycerate mutase family member 5BXLBV68, serine/threonine-protein phosphatase PGAM5, mitochondrial

Gene Symbol

PGAM5

UniProt

Q96HS1 (Human)

Additional PGAM5 Products

Product Documents for PGAM5 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for PGAM5 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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