PGK1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-19784

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown, Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat, Yeast

Predicted:

Bovine (96%), Chimpanzee (100%), Porcine (96%), Rhesus Macaque (100%), Sheep (96%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Proximity Ligation Assay

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all PGK1 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human PGK1. The exact sequence is proprietary.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Zebrafish (87%), Xenopus laevis (84%), Chicken (88%).

Localization

Cytoplasm

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

45 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PGK1 Antibody - BSA Free

Immunohistochemistry-Paraffin: PGK1 Antibody [NBP2-19784]

Immunohistochemistry-Paraffin: PGK1 Antibody [NBP2-19784]

Immunohistochemistry-Paraffin: PGK1 Antibody [NBP2-19784] - Analysis of paraffin-embedded hepatoma, using antibody at 1:500 dilution.
Western Blot: PGK1 Antibody [NBP2-19784]

Western Blot: PGK1 Antibody [NBP2-19784]

Western Blot: PGK1 Antibody [NBP2-19784] - PGK1 antibody detects PGK1 protein by Western blot analysis. 50 ug Rat liver lysate/extract, 10% SDS-PAGE, PGK1 antibody dilution: 1:1000.
Western Blot: PGK1 Antibody [NBP2-19784]

Western Blot: PGK1 Antibody [NBP2-19784]

Western Blot: PGK1 Antibody [NBP2-19784] - PGK1 antibody detects PGK1 protein in 1% O2-treated samples by Western blot analysis. Upper panel: PGK1 antibody. Lower panel: beta Actin antibody. A: 30 ug HeLa whole cell lysate/extract (untreated). B: 30 ug HeLa whole cell lysate/extract (1% O2 treatment for 24 hours).
Western Blot: PGK1 Antibody [NBP2-19784]

Western Blot: PGK1 Antibody [NBP2-19784]

Western Blot: PGK1 Antibody [NBP2-19784] - 50 ug mouse brain whole cell lysate, 10% SDS PAGE, antibody at 1:1000.
Immunocytochemistry/ Immunofluorescence: PGK1 Antibody [NBP2-19784]

Immunocytochemistry/ Immunofluorescence: PGK1 Antibody [NBP2-19784]

Immunocytochemistry/Immunofluorescence: PGK1 Antibody [NBP2-19784] - Analysis of methanol-fixed HeLa, using antibody at 1:50 dilution.
PGK1 Antibody

Western Blot: PGK1 Antibody [NBP2-19784] -

Western Blot: PGK1 Antibody [NBP2-19784] - Non-transfected (–) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PGK1 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
PGK1 Antibody - BSA Free

Western Blot: PGK1 Antibody [NBP2-19784] -

PGK1 antibody detects PGK1 protein by western blot analysis. Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PGK1 antibody (NBP2-19784) diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
PGK1 Antibody - BSA Free

Western Blot: PGK1 Antibody [NBP2-19784] -

Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PGK1 antibody (NBP2-19784) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
PGK1 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: PGK1 Antibody [NBP2-19784] -

PGK1 antibody detects PGK1 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: PGK1 protein stained by PGK1 antibody (NBP2-19784) diluted at 1:20.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [B-5-1-2] diluted at 1:10000.
Blue: Hoechst 33342 staining.

Applications for PGK1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:20-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Immunoprecipitation

Assay dependent

Proximity Ligation Assay

Assay dependent

Western Blot

1:500-1:3000

Reviewed Applications

Read 1 review rated 5 using NBP2-19784 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 20% Glycerol

Format

BSA Free

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: PGK1

The protein encoded by this gene is a glycolytic enzyme that catalyzes the conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate. The encoded protein may also act as a cofactor for polymerase alpha. This gene lies on the X-chromosome, while a related pseudogene also has been found on the X-chromosome and another on chromosome 19. [provided by RefSeq]

Long Name

Phosphoglycerate Kinase-1

Alternate Names

MIG10, PGKA, PRP2

Gene Symbol

PGK1

UniProt

P00558

Additional PGK1 Products

Product Documents for PGK1 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for PGK1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for PGK1 Antibody - BSA Free

Customer Reviews for PGK1 Antibody - BSA Free (1)

5 out of 5
1 Customer Rating
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  • PGK1 Antibody
    Name: Min-Joon Han
    Application: Western Blot
    Sample Tested: Human fibroblast and human iPSC
    Species: Human
    Verified Customer | Posted 08/16/2018
    lane #1: human fibroblast cell lane #2: human iPSC
    PGK1 Antibody - BSA Free NBP2-19784

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Protocols

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