PGK2 Antibody - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-94204

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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free
View all PGK2 Primary Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 242-360 of human PGK2 (NP_620061.2). YTFLKVLNNMEIGASLFDEEGAKIVKDIMAKAQKNGVRITFPVDFVTGDKFDENAQVGKATVASGISPGWMGLDCGPESNKNHAQVVAQARLIVWNGPLGVFEWDAFAKGTKALMDEIV

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

44 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PGK2 Antibody - Azide and BSA Free

Western Blot: PGK2 AntibodyAzide and BSA Free [NBP2-94204]

Western Blot: PGK2 AntibodyAzide and BSA Free [NBP2-94204]

Western Blot: PGK2 Antibody [NBP2-94204] - Analysis of extracts of various cell lines, using PGK2 at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.
PGK2 Antibody - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: PGK2 Antibody - Azide and BSA Free [PGK2] -

Immunocytochemistry/ Immunofluorescence: PGK2 Antibody - Azide and BSA Free [PGK2] - Immunofluorescence analysis of C6 cells using PGK2 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunocytochemistry/ Immunofluorescence: PGK2 Antibody - Azide and BSA Free [NBP2-94204]

Immunocytochemistry/ Immunofluorescence: PGK2 Antibody - Azide and BSA Free [NBP2-94204]

Immunocytochemistry/Immunofluorescence: PGK2 Antibody [NBP2-94204] - Analysis of L929 cells using PGK2. Blue: DAPI for nuclear staining.
Immunohistochemistry-Paraffin: PGK2 Antibody - Azide and BSA Free [NBP2-94204]

Immunohistochemistry-Paraffin: PGK2 Antibody - Azide and BSA Free [NBP2-94204]

Immunohistochemistry-Paraffin: PGK2 Antibody [NBP2-94204] - Paraffin-embedded rat testis using PGK2.
Immunohistochemistry-Paraffin: PGK2 Antibody - Azide and BSA Free [NBP2-94204]

Immunohistochemistry-Paraffin: PGK2 Antibody - Azide and BSA Free [NBP2-94204]

Immunohistochemistry-Paraffin: PGK2 Antibody [NBP2-94204] - Paraffin-embedded human mammary cancer using PGK2.
Immunohistochemistry-Paraffin: PGK2 Antibody - Azide and BSA Free [NBP2-94204]

Immunohistochemistry-Paraffin: PGK2 Antibody - Azide and BSA Free [NBP2-94204]

Immunohistochemistry-Paraffin: PGK2 Antibody [NBP2-94204] - Paraffin-embedded mouse testis using PGK2.

Applications for PGK2 Antibody - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:100

Immunohistochemistry

1:50-1:100

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

Azide and BSA Free

Preservative

0.01% Thimerosal

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PGK2

The PGK2 gene encodes a testis-specific form of phosphoglycerate kinase (EC 2.7.2.3), which catalyzes the reversible conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate during glycolysis, generating one molecule of ATP. See also PGK1 (MIM 311800), which is ubiquitously expressed in all somatic tissues and maps to chromosome Xq13.[supplied by OMIM]

Alternate Names

dJ417L20.2, EC 2.7.2.3, PGK-2, PGKB, PGKPS, phosphoglycerate kinase 2, phosphoglycerate kinase autosomal pseudogene, Phosphoglycerate kinase, testis specific

Gene Symbol

PGK2

Additional PGK2 Products

Product Documents for PGK2 Antibody - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for PGK2 Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

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Protocols

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