PGM3 Antibody (1E2-1B12) - Azide and BSA Free

Novus Biologicals | Catalog # H00005238-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Sandwich ELISA, Immunoprecipitation, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 1E2-1B12

Format

Azide and BSA Free
View all PGM3 Primary Antibodies »

Product Specifications

Immunogen

PGM3 (AAH01258, 1 a.a. ~ 542 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MDLGAITKYSALHAKPNGLILQYGTAGFRTKAEHLDHVMFRMGLLAVLRSKQTKSTIGVMVTASHNPEEDNGVKLVDPLGEMLAPSWEEHATCLANAEEQDMQRVLIDISEKEAVNLQQDAFVVIGRDTRPSSEKLSQSVIDGVTVLGGQFHDYGLLTTPQLHYMVYCRNTGGRYGKATIEGYYQKLSKAFVELTKQASCSGDEYRSLKVDCANGIGALKLREMEHYFSQGLSVQLFNDGSKGKLNHLCGADFVKSHQKPPQGMEIKSNERCCSFDGDADRIVYYYHDADGHFHLIDGDKIATLISSFLKELLVEIGESLNIGVVQTAYANGSSTRYLEEVMKVPVYCTKTGVKHLHHKAQEFDIGVYFEANGHGTALFSTAVEMKIKQSAEQLEDKKRKAAKMLENIIDLFNQAAGDAISDMLVIEAILALKGLTVQQWDALYTDLPNRQLKVQVADRRVISTTNAERQAVTPPGLQEAINDLVKKYKLSRAFVRPSGTEDVVRVYAEADSQESADHLAHEVSLAVFQLAGGIGERPQPGF

Specificity

PGM3 - phosphoglucomutase 3

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for PGM3 Antibody (1E2-1B12) - Azide and BSA Free

Western Blot: PGM3 Antibody (1E2-1B12) [H00005238-M01]

Western Blot: PGM3 Antibody (1E2-1B12) [H00005238-M01]

Western Blot: PGM3 Antibody (1E2-1B12) [H00005238-M01] - Analysis of PGM3 expression in transfected 293T cell line by PGM3 monoclonal antibody (M01), clone 1E2-1B12.Lane 1: PGM3 transfected lysate(59.9 KDa).Lane 2: Non-transfected lysate.
Immunohistochemistry-Paraffin: PGM3 Antibody (1E2-1B12) [H00005238-M01]

Immunohistochemistry-Paraffin: PGM3 Antibody (1E2-1B12) [H00005238-M01]

Immunohistochemistry-Paraffin: PGM3 Antibody (1E2-1B12) [H00005238-M01] - Analysis of monoclonal antibody to PGM3 on formalin-fixed paraffin-embedded human colon tissue. Antibody concentration 2 ug/ml.
Western Blot: PGM3 Antibody (1E2-1B12) [H00005238-M01]

Western Blot: PGM3 Antibody (1E2-1B12) [H00005238-M01]

Western Blot: PGM3 Antibody (1E2-1B12) [H00005238-M01] - Analysis of PGM3 over-expressed 293 cell line, cotransfected with PGM3 Validated Chimera RNAi ( Cat # H00005238-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with PGM3 monoclonal antibody (M01), clone 1E2-1B12 (Cat # H00005238-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Western Blot: PGM3 Antibody (1E2-1B12) [H00005238-M01]

Western Blot: PGM3 Antibody (1E2-1B12) [H00005238-M01]

Western Blot: PGM3 Antibody (1E2-1B12) [H00005238-M01] - PGM3 monoclonal antibody (M01), clone 1E2-1B12 Analysis of PGM3 expression in HeLa.
Immunoprecipitation: PGM3 Antibody (1E2-1B12) [H00005238-M01]

Immunoprecipitation: PGM3 Antibody (1E2-1B12) [H00005238-M01]

Immunoprecipitation: PGM3 Antibody (1E2-1B12) [H00005238-M01] - Analysis of PGM3 transfected lysate using anti-PGM3 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PGM3 MaxPab rabbit polyclonal antibody.
Sandwich ELISA: PGM3 Antibody (1E2-1B12) [H00005238-M01] - Detection limit for recombinant GST tagged PGM3 is approximately 3ng/ml as a capture antibody.

Applications for PGM3 Antibody (1E2-1B12) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IHC-P, RNAi Validation and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: PGM3

Interconverts GlcNAc-6-P and GlcNAc-1-P

Alternate Names

AGM1PAGMAcetylglucosamine phosphomutase, DKFZP434B187, EC 5.4.2, EC 5.4.2.3, FLJ11614, FLJ13623, N-acetylglucosamine-phosphate mutase, N-acetylglucosamine-phosphate mutase 1, PGM 3, phosphoacetylglucosamine mutase, phosphoglucomutase 3, Phosphoglucomutase-3

Entrez Gene IDs

5238 (Human)

Gene Symbol

PGM3

OMIM

172100 (Human)

UniProt

AAH01258 (Human)

Additional PGM3 Products

Product Documents for PGM3 Antibody (1E2-1B12) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for PGM3 Antibody (1E2-1B12) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for PGM3 Antibody (1E2-1B12) - Azide and BSA Free

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Protocols

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FAQs

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