Phenylalanine Hydroxylase Antibody - BSA Free

Novus Biologicals | Catalog # NBP3-35577

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Phenylalanine Hydroxylase Primary Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-290 of human Phenylalanine Hydroxylase (NP_000268.1).

Sequence:
MSTAVLENPGLGRKLSDFGQETSYIEDNCNQNGAISLIFSLKEEVGALAKVLRLFEENDVNLTHIESRPSRLKKDEYEFFTHLDKRSLPALTNIIKILRHDIGATVHELSRDKKKDTVPWFPRTIQELDRFANQILSYGAELDADHPGFKDPVYRARRKQFADIAYNYRHGQPIPRVEYMEEEKKTWGTVFKTLKSLYKTHACYEYNHIFPLLEKYCGFHEDNIPQLEDVSQFLQTCTGFRLRPVAGLLSSRDFLGGLAFRVFHCTQYIRHGSKPMYTPEPDICHELLGH

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

52 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Phenylalanine Hydroxylase Antibody - BSA Free

Phenylalanine Hydroxylase Antibody

Western Blot: Phenylalanine Hydroxylase Antibody [NBP3-35577] -

Western Blot: Phenylalanine Hydroxylase Antibody [NBP3-35577] - Western blot analysis of lysates from HepG2 cells, using Phenylalanine Hydroxylase Rabbit pAb at 1:700 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.
Phenylalanine Hydroxylase Antibody

Immunocytochemistry/ Immunofluorescence: Phenylalanine Hydroxylase Antibody [NBP3-35577] -

Immunocytochemistry/ Immunofluorescence: Phenylalanine Hydroxylase Antibody [NBP3-35577] - Immunofluorescence analysis of HepG2 cells using Phenylalanine Hydroxylase Rabbit pAb at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Applications for Phenylalanine Hydroxylase Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Phenylalanine Hydroxylase

PAH encodes the enzyme phenylalanine hydroxylase that is the rate-limiting step in phenylalanine catabolism. Deficiency of this enzyme activity results in the autosomal recessive disorder phenylketonuria.

Alternate Names

EC 1.14.16, EC 1.14.16.1, PH, phe-4-monooxygenase, phenylalanine 4-monooxygenase, phenylalanine hydroxylase, phenylalanine-4-hydroxylase, PKU, PKU1

Gene Symbol

PAH

Additional Phenylalanine Hydroxylase Products

Product Documents for Phenylalanine Hydroxylase Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Phenylalanine Hydroxylase Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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