PHGDH Antibody (CL0555) - BSA Free

Novus Biologicals | Catalog # NBP2-52939

Novus Biologicals
100% Guarantee

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Predicted:

Mouse (99%), Rat (99%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL0555

Format

BSA Free
View all PHGDH Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed using a recombinant protein derived from O43175, with the exact immunogen sequence remaining proprietary.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for PHGDH Antibody (CL0555) - BSA Free

Western Blot: PHGDH Antibody (CL0555) [NBP2-52939] -

Western Blot: PHGDH Antibody (CL0555) [NBP2-52939] - Lane 1: Marker [kDa], Lane 2: Human cell line RT-4
Immunocytochemistry/ Immunofluorescence: PHGDH Antibody (CL0555) [NBP2-52939]

Immunocytochemistry/ Immunofluorescence: PHGDH Antibody (CL0555) [NBP2-52939]

Immunocytochemistry/Immunofluorescence: PHGDH Antibody (CL0555) [NBP2-52939] - Staining of MCF7 cells using the Anti-PHGDH monoclonal antibody, showing specific staining in the cytosol and the plasma membrane in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).
PHGDH Antibody (CL0555)

Immunohistochemistry-Paraffin: PHGDH Antibody (CL0555) [NBP2-52939] -

Immunohistochemistry-Paraffin: PHGDH Antibody (CL0555) [NBP2-52939] -Analysis in human pancreas and skeletal muscle tissues using NBP2-52939 antibody. Corresponding PHGDH RNA-seq data are presented for the same tissues.
Western Blot: PHGDH Antibody (CL0555) [NBP2-52939]

Western Blot: PHGDH Antibody (CL0555) [NBP2-52939]

Western Blot: PHGDH Antibody (CL0555) [NBP2-52939] - Lane 1: Marker [kDa], Lane 2: Human cell line HeLa cytoplasmic fraction, Lane 3: Human cell line HeLa membrane fraction, Lane 4: Human cell line HeLa nuclear fraction, Lane 5: Human cell line HeLa chromatin fraction, Lane 6: Human cell line HeLa cytos
PHGDH Antibody (CL0555)

Immunohistochemistry-Paraffin: PHGDH Antibody (CL0555) [NBP2-52939] -

Immunohistochemistry-Paraffin: PHGDH Antibody (CL0555) [NBP2-52939] -Staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.
PHGDH Antibody (CL0555)

Immunohistochemistry-Paraffin: PHGDH Antibody (CL0555) [NBP2-52939] -

Immunohistochemistry-Paraffin: PHGDH Antibody (CL0555) [NBP2-52939] -Staining of human liver shows strong cytoplasmic positivity in hepatocytes.
PHGDH Antibody (CL0555)

Immunohistochemistry-Paraffin: PHGDH Antibody (CL0555) [NBP2-52939] -

Immunohistochemistry-Paraffin: PHGDH Antibody (CL0555) [NBP2-52939] -Staining of human skin shows strong cytoplasmic positivity in squamous epithelial cells.
PHGDH Antibody (CL0555)

Immunohistochemistry-Paraffin: PHGDH Antibody (CL0555) [NBP2-52939] -

Immunohistochemistry-Paraffin: PHGDH Antibody (CL0555) [NBP2-52939] -Staining of human skeletal muscle shows no positivity in myocytes as expected.

Applications for PHGDH Antibody (CL0555) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. For ICC/IF, PFA/Triton X-100 is recommended for fixation/permeabilization.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PHGDH

3-Phosphoglycerate dehydrogenase (PHGDH; EC 1.1.1.95) catalyzes the transition of 3-phosphoglycerate into 3-phosphohydroxypyruvate, which is the first and rate-limiting step in the phosphorylated pathway of serine biosynthesis, using NAD+/NADH as a cofactor.[supplied by OMIM]

Long Name

3-Phosphoglycerate Dehydrogenase

Alternate Names

3PGDH, PDGH3

Gene Symbol

PHGDH

Additional PHGDH Products

Product Documents for PHGDH Antibody (CL0555) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for PHGDH Antibody (CL0555) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Redox Enzymes

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Protocols

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FAQs

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