PHGDH Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-32201

Novus Biologicals
100% Guarantee

Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Bovine (97%), Chimpanzee (100%), Porcine (97%). Backed by our 100% Guarantee.

Applications

Knockout Validated, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all PHGDH Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human PHGDH. The exact sequence is proprietary.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

57 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Knockout (KO) Validated Rabbit PHGDH Antibody - BSA Free (NBP1-32201) is a polyclonal antibody validated for use in IHC, WB, ICC/IF and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for PHGDH Antibody - BSA Free

Knockout Validated: PHGDH Antibody [NBP1-32201]

Western Blot: PHGDH Antibody [NBP1-32201]

Western Blot: PHGDH Antibody [NBP1-32201] - Wild-type (WT) and PHGDH knockout (KO) HeLa cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PHGDH antibody [N1N2], N-term. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Western Blot: PHGDH Antibody [NBP1-32201]

Western Blot: PHGDH Antibody [NBP1-32201]

Western Blot: PHGDH Antibody [NBP1-32201] - Sample(30 ug of whole cell lysate)A:293TB:A431 C:HeLa S3 7. 5% SDS PAGE, antibody diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: PHGDH Antibody [NBP1-32201]

Immunocytochemistry/ Immunofluorescence: PHGDH Antibody [NBP1-32201]

Immunocytochemistry/Immunofluorescence: PHGDH Antibody [NBP1-32201] - PHGDH antibody detects PHGDH protein by immunohistochemical analysis. Sample: Frozen-sectioned rat E13.5 brain. Green: PHGDH stained by PHGDH antibody diluted at 1:250. Blue: Fluoroshield with DAPI.
Immunohistochemistry-Paraffin: PHGDH Antibody [NBP1-32201]

Immunohistochemistry-Paraffin: PHGDH Antibody [NBP1-32201]

Immunohistochemistry-Paraffin: PHGDH Antibody [NBP1-32201] - Paraffin-embedded mouse pancreas. PHGDH antibody [N1N2], N-term diluted at 1:500.
Western Blot: PHGDH Antibody [NBP1-32201]

Western Blot: PHGDH Antibody [NBP1-32201]

Western Blot: PHGDH Antibody [NBP1-32201] - Sample (50 ug of whole cell lysate) A: Mouse brain 7. 5% SDS PAGE; antibody diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: PHGDH Antibody [NBP1-32201]

Immunocytochemistry/ Immunofluorescence: PHGDH Antibody [NBP1-32201]

Immunocytochemistry/Immunofluorescence: PHGDH Antibody [NBP1-32201] - Analysis of methanol-fixed HeLa, using PHGDH antibody at 1:500 dilution.
Immunohistochemistry-Paraffin: PHGDH Antibody [NBP1-32201]

Immunohistochemistry-Paraffin: PHGDH Antibody [NBP1-32201]

Immunohistochemistry-Paraffin: PHGDH Antibody [NBP1-32201] - Paraffin-embedded PC13 xenograft, using antibody at 1:500 dilution.
Immunoprecipitation: PHGDH Antibody [NBP1-32201]

Immunoprecipitation: PHGDH Antibody [NBP1-32201]

Immunoprecipitation: PHGDH Antibody [NBP1-32201] - Immunoprecipitation of PHGDH protein from 293T whole cell extracts using 5 ug of PHGDH antibody [N1N2], N-term (NBP1-32201). Western blot analysis was performed using PHGDH antibody [N1N2], N-term (NBP1-32201). EasyBlot anti-Rabbit IgG was used as a secondary reagent.

Applications for PHGDH Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Frozen

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Immunoprecipitation

1:100-1:500

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris, 0.1M Glycine, 10% Glycerol

Format

BSA Free

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: PHGDH

3-Phosphoglycerate dehydrogenase (PHGDH; EC 1.1.1.95) catalyzes the transition of 3-phosphoglycerate into 3-phosphohydroxypyruvate, which is the first and rate-limiting step in the phosphorylated pathway of serine biosynthesis, using NAD+/NADH as a cofactor.[supplied by OMIM]

Long Name

3-Phosphoglycerate Dehydrogenase

Alternate Names

3PGDH, PDGH3

Entrez Gene IDs

26227 (Human)

Gene Symbol

PHGDH

UniProt

O43175 (Human)

Additional PHGDH Products

Product Documents for PHGDH Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for PHGDH Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Related Research Areas

Redox Enzymes

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Protocols

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FAQs

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