Phosphatase Activity Assays & Reagents

DuoSet® IC (Intracellular) Phosphatase Activity Assays

A sensitive and highly selective alternative to single substrate phosphatase assays, these kits contain capture antibody, phospho-peptide substrate, and Malachite green for detecting inorganic phosphate. The target phosphatase is bound by the capture antibody. The captured phosphatase liberates phosphate from the phospho-peptide substrate and the free phosphate is measured by a change in color of Malachite green. Kits are available in 2-, 5-, and 15-(96-well) plate pack sizes.

Kit Contents:*

Assay Principle

View Full Assay Principle
  • Capture Antibody
  • Phosphatase Substrate
  • Phosphate Standard
  • Malachite Green Reagent A & B

*Kit contents may vary slightly. Please refer to product-specific inserts for a complete list of contents for individual assays.

Phosphatase Peptide Substrates

R&D Systems offers several synthetic phospho-peptide substrates that can be used for measuring the activity of serine/threonine or tyrosine phosphatases including PP1, PP2A, PP2B (Calcineurin), PP2C, PTP1B, Yersinia PTP, SHP-2, and more. Phosphatase-induced liberation of inorganic phosphate can be measured using the Malachite Green Phosphate Detection Kit (Catalog # DY996).