Phospho-Akt (S473) PE-conjugated Antibody

Applications

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Intracellular Staining by Flow Cytometry View Larger

Detection of Phospho-Akt (S473) in Jurkat Human Cell Line by Flow Cytometry. Jurkat human acute T cell leukemia cell line either untreated (open histogram) or treated with 100 nM Calyculin A (Catalog # 1336) for 30 minutes (filled histogram) was stained with Mouse Anti-Human/Mouse Phospho-Akt (S473) PE-conjugated Monoclonal Antibody (Catalog # IC7794P). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Preparation and Storage

Background: Akt

Akt, also known as Protein Kinase B (PKB), is a central kinase in such diverse cellular processes as glucose uptake, cell cycle progression, and apoptosis. Three highly homologous members define the Akt family: Akt1 (PKB alpha ), Akt2 (PKB beta ), and Akt3 (PKB gamma ). All three Akts contain an amino-terminal pleckstrin homology domain, a central kinase domain, and a carboxyl-terminal regulatory domain. Akt1 is the most widely expressed family member and is frequently activated in a number of carcinomas, including breast, prostate, lung, pancreatic, liver, ovarian, and colorectal cancer. Akt1 is activated in a multistep process that involves the sequential phosphorylation of Thr450 by JNK kinases, Thr308 by PDK1, and Ser473 by PDK2 or mTORC2. Activated Akt1 phosphorylates a wide variety of cytosolic, nuclear, and mitochondrial substrates. Human Akt1 shares 98% aa sequence identity with mouse and rat Akt1.

Product Datasheets

Related Research Areas

• Apoptosis Intracellular Kinases
• Cancer Biomarkers
• Chemokine Receptor Intracellular Signaling
• Common gamma Chain Receptor Family
• Diabetic Peripheral Neuropathy
• GDNF Family
• IL-10 Family
• Interferons
• Intracellular Kinases
• Intracellular Kinases in the Akt Pathway
• Intracellular Signaling Molecules in Angiogenesis
• Kinases and Phosphatases in Endothelial Cells
• Multiple Myeloma
• Neurotrophin/Trk Family
• Notch Pathway
• PDGF Family
• Platelet Signaling
• Search Kinases and Phosphatases
• Signaling Molecules and Transcriptional Regulators of Tfh Cell Differentiation
• VEGF Family