Phospho-Akt (S473) Alexa Fluor® 488-conjugated Antibody

Catalog # Availability Size / Price Qty
IC7794G
Phospho-Akt (S473) Alexa Fluor® 488-conjugated Antibody in Intracellular Staining by Flow Cytometry
1 Image
Product Details
FAQs
Supplemental Products
Reviews

Phospho-Akt (S473) Alexa Fluor® 488-conjugated Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects human and mouse Akt1, Akt2 and Akt3, when phosphorylated at S473, S474 and S472, respectively.
Source
Monoclonal Mouse IgG1 Clone # 545007
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Phosphopeptide containing the human Akt (S473) site
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 488

Applications

Recommended Concentration
Sample
Intracellular Staining by Flow Cytometry
5 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Intracellular Staining by Flow Cytometry Detection of Phospho-Akt (S473) in Jurkat Human Cell Line by Flow Cytometry. View Larger

Detection of Phospho-Akt (S473) in Jurkat Human Cell Line by Flow Cytometry. Jurkat human acute T cell leukemia cell line either untreated (open histogram), or treated with 100 nM Calyculin A (Catalog # 1336) for 30 min (filled histogram), was stained with Mouse Anti-Human/Mouse Phospho-Akt (S473) Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # IC7794G). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Akt

Akt, also known as Protein Kinase B (PKB), is a central kinase in such diverse cellular processes as glucose uptake, cell cycle progression, and apoptosis. Three highly homologous members define the Akt family: Akt1 (PKB alpha ), Akt2 (PKB beta ), and Akt3 (PKB gamma ). All three Akts contain an amino-terminal pleckstrin homology domain, a central kinase domain, and a carboxyl-terminal regulatory domain. Akt1 is the most widely expressed family member and is frequently activated in a number of carcinomas, including breast, prostate, lung, pancreatic, liver, ovarian, and colorectal cancer. Akt1 is activated in a multistep process that involves the sequential phosphorylation of Thr450 by JNK kinases, Thr308 by PDK1, and Ser473 by PDK2 or mTORC2. Activated Akt1 phosphorylates a wide variety of cytosolic, nuclear, and mitochondrial substrates. Human Akt1 shares 98% aa sequence identity with mouse and rat Akt1.

Long Name
v-Akt Murine Thymoma Viral Oncogene Homolog
Alternate Names
RAC-ALPHA; AKT serine/threonine kinase 1; Akt; PKB; PKB-ALPHA; PRKBA; RAC

Product Datasheets

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Reviews for Phospho-Akt (S473) Alexa Fluor® 488-conjugated Antibody

There are currently no reviews for this product. Be the first to review Phospho-Akt (S473) Alexa Fluor® 488-conjugated Antibody and earn rewards!

Have you used Phospho-Akt (S473) Alexa Fluor® 488-conjugated Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review