PI 4 Kinase type 2 beta Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-87908

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all PI 4 Kinase type 2 beta Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: LILPYISDMNFVQDLCEDLYELFKTDKGFDKATFESQMSVMRGQILNLTQALRDGKSPFQLVQIPCVIVERSQGGSQGRIVHLSNSFTQTVNCRKPF

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (87%), Rat (86%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PI 4 Kinase type 2 beta Antibody - BSA Free

PI 4 Kinase type 2 beta Antibody - BSA Free Immunocytochemistry/ Immunofluorescence: PI 4 Kinase type 2 beta Antibody - BSA Free [NBP1-87908]

Immunocytochemistry/ Immunofluorescence: PI 4 Kinase type 2 beta Antibody - BSA Free [NBP1-87908]

Staining of human cell line A-431 shows localization to cytosol.
Immunohistochemistry-Paraffin: PI 4 Kinase type 2 beta Antibody [NBP1-87908]

Immunohistochemistry-Paraffin: PI 4 Kinase type 2 beta Antibody [NBP1-87908]

Immunohistochemistry-Paraffin: PI 4 Kinase type 2 beta Antibody [NBP1-87908] - Staining of human prostate shows moderate cytoplasmic positivity in smooth muscle cells.
Immunohistochemistry-Paraffin: PI 4 Kinase type 2 beta Antibody [NBP1-87908]

Immunohistochemistry-Paraffin: PI 4 Kinase type 2 beta Antibody [NBP1-87908]

Immunohistochemistry-Paraffin: PI 4 Kinase type 2 beta Antibody [NBP1-87908] - Staining of human duodenum shows moderate to strong membranous positivity in glandular cells.
Immunohistochemistry-Paraffin: PI 4 Kinase type 2 beta Antibody [NBP1-87908]

Immunohistochemistry-Paraffin: PI 4 Kinase type 2 beta Antibody [NBP1-87908]

Immunohistochemistry-Paraffin: PI 4 Kinase type 2 beta Antibody [NBP1-87908] - Staining of human lymph node shows moderate to strong membranous positivity in non-germinal and germinal center cells.
Immunohistochemistry-Paraffin: PI 4 Kinase type 2 beta Antibody [NBP1-87908]

Immunohistochemistry-Paraffin: PI 4 Kinase type 2 beta Antibody [NBP1-87908]

Immunohistochemistry-Paraffin: PI 4 Kinase type 2 beta Antibody [NBP1-87908] - Staining of human pancreas shows very weak positivity in exocrine glandular cells as expected.
PI 4 Kinase type 2 beta Antibody - BSA Free Western Blot: PI 4 Kinase type 2 beta Antibody - BSA Free [NBP1-87908]

Western Blot: PI 4 Kinase type 2 beta Antibody - BSA Free [NBP1-87908]

Analysis in control (vector only transfected HEK293T lysate) and PI4K2B over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells).

Applications for PI 4 Kinase type 2 beta Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PI 4 Kinase type 2 beta

Phosphatidylinositol 4-kinases (PI4Ks) phosphorylate phosphatidylinositol to generate phosphatidylinositol 4-phosphate (PIP), an immediate precursor of several important signaling and scaffolding molecules. PIP itself may also have direct functional and structural roles. PI4K2B is a primarily cytosolic PI4K that is recruited to membranes, where it stimulates phosphatidylinositol 4,5-bisphosphate synthesis (Wei et al., 2002 (PubMed 12324459)).(supplied by OMIM)

Long Name

Phosphatidylinositol 4-kinase type 2-beta

Alternate Names

EC 2.7.1.67, PI4K2B, PI4KII-BETA

Gene Symbol

PI4K2B

Additional PI 4 Kinase type 2 beta Products

Product Documents for PI 4 Kinase type 2 beta Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for PI 4 Kinase type 2 beta Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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