PIAS2 Antibody (1F7) - Azide and BSA Free

Novus Biologicals | Catalog # H00009063-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Sandwich ELISA, Immunocytochemistry/ Immunofluorescence, Proximity Ligation Assay, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 1F7

Format

Azide and BSA Free
View all PIAS2 Primary Antibodies »

Product Specifications

Immunogen

PIAS2 (NP_004662, 385 a.a. ~ 473 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. CPVCDKKAAYESLILDGLFMEILNDCSDVDEIKFQEDGSWCPMRPKKEAMKVSSQPCTKIESSSVLSKPCSVTVASEASKKKVDVIDLT

Reactivity Notes

Human. Other species not tested.

Localization

Nuclear

Specificity

PIAS2 - protein inhibitor of activated STAT, 2

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for PIAS2 Antibody (1F7) - Azide and BSA Free

Western Blot: PIAS2 Antibody (1F7) [H00009063-M01]

Western Blot: PIAS2 Antibody (1F7) [H00009063-M01]

Western Blot: PIAS2 Antibody (1F7) [H00009063-M01] - Analysis of PIAS2 over-expressed 293 cell line, cotransfected with PIAS2 Validated Chimera RNAi ( Cat # H00009063-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with PIAS2 monoclonal antibody (M01), clone 1F7 (Cat # H00009063-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Immunocytochemistry/ Immunofluorescence: PIAS2 Antibody (1F7) [H00009063-M01]

Immunocytochemistry/ Immunofluorescence: PIAS2 Antibody (1F7) [H00009063-M01]

Immunocytochemistry/Immunofluorescence: PIAS2 Antibody (1F7) [H00009063-M01] - Analysis of monoclonal antibody to PIAS2 on HeLa cell. Antibody concentration 10 ug/ml.
Proximity Ligation Assay: PIAS2 Antibody (1F7) [H00009063-M01]

Proximity Ligation Assay: PIAS2 Antibody (1F7) [H00009063-M01]

Proximity Ligation Assay: PIAS2 Antibody (1F7) [H00009063-M01] - Analysis of protein-protein interactions between TP53 and PIAS2. HeLa cells were stained with anti-TP53 rabbit purified polyclonal 1:1200 and anti-PIAS2 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
Sandwich ELISA: PIAS2 Antibody (1F7) [H00009063-M01] - Detection limit for recombinant GST tagged PIAS2 is approximately 0.3ng/ml as a capture antibody.

Applications for PIAS2 Antibody (1F7) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactive against Recombinant Protein with GST tag on ELISA and Western Blot. GST tag alone is used as a negative control. Has also been used for RNAi validation and immunofluorescence.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: PIAS2

This gene encodes a protein involved in the regulation of transcription factors involved in MAP kinase signaling. The symbol MIZ1 has also been associated with ZBTB17 which is a different gene located on chromosome 1. Two alternatively spliced transcripts encoding different isoforms have been described.

Long Name

Protein Inhibitor of Activated STAT 2

Alternate Names

ARIP3, DIP, MIZ1, PIASx alpha, SIZ2, ZMIZ4

Entrez Gene IDs

9063 (Human)

Gene Symbol

PIAS2

OMIM

603567 (Human)

UniProt

NP_004662 (Human)

Additional PIAS2 Products

Product Documents for PIAS2 Antibody (1F7) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for PIAS2 Antibody (1F7) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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