PICALM Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-86659

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation, Independent Antibodies

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all PICALM Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: VHLSISSDVSTFTTRTPTHEMFVGFTPSPVAQPHPSAGLNVDFESVFGNKSTNVIVDSGGFDELGGLLKPTVASQNQNLPVAKLPPSKLVSDDLDSSLA

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PICALM Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: PICALM Antibody [NBP1-86659]

Immunocytochemistry/ Immunofluorescence: PICALM Antibody [NBP1-86659]

Immunocytochemistry/Immunofluorescence: PICALM Antibody [NBP1-86659] - Staining of human cell line U-251 MG shows localization to vesicles.
Immunohistochemistry-Paraffin: PICALM Antibody [NBP1-86659]

Immunohistochemistry-Paraffin: PICALM Antibody [NBP1-86659]

Immunohistochemistry-Paraffin: PICALM Antibody [NBP1-86659] - Staining in human placenta and skeletal muscle tissues using NBP1-86659 antibody. Corresponding PICALM RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: PICALM Antibody [NBP1-86659]

Immunohistochemistry-Paraffin: PICALM Antibody [NBP1-86659]

Immunohistochemistry-Paraffin: PICALM Antibody [NBP1-86659] - Staining of human cerebellum shows moderate cytoplasmic positivity in Purkinje cells.
Immunohistochemistry-Paraffin: PICALM Antibody [NBP1-86659]

Immunohistochemistry-Paraffin: PICALM Antibody [NBP1-86659]

Immunohistochemistry-Paraffin: PICALM Antibody [NBP1-86659] - Staining of human cerebellum, duodenum, placenta and skeletal muscle using Anti-PICALM antibody NBP1-86659 (A) shows similar protein distribution across tissues to independent antibody NBP1-86658 (B).
Immunohistochemistry-Paraffin: PICALM Antibody [NBP1-86659]

Immunohistochemistry-Paraffin: PICALM Antibody [NBP1-86659]

Immunohistochemistry-Paraffin: PICALM Antibody [NBP1-86659] - Staining of human duodenum shows strong membranous and cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: PICALM Antibody [NBP1-86659]

Immunohistochemistry-Paraffin: PICALM Antibody [NBP1-86659]

Immunohistochemistry-Paraffin: PICALM Antibody [NBP1-86659] - Staining of human placenta shows strong membranous and cytoplasmic positivity in trophoblastic cells.
Immunohistochemistry-Paraffin: PICALM Antibody [NBP1-86659]

Immunohistochemistry-Paraffin: PICALM Antibody [NBP1-86659]

Immunohistochemistry-Paraffin: PICALM Antibody [NBP1-86659] - Staining of human skeletal muscle shows no positivity in myocytes as expected.
PICALM Antibody - BSA Free Western Blot: PICALM Antibody - BSA Free [NBP1-86659]

Western Blot: PICALM Antibody - BSA Free [NBP1-86659]

Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
PICALM Antibody - BSA Free Western Blot: PICALM Antibody - BSA Free [NBP1-86659]

Western Blot: PICALM Antibody - BSA Free [NBP1-86659]

Analysis in human cell lines Caco-2 and PC-3 using Anti-PICALM antibody. Corresponding PICALM RNA-seq data are presented for the same cell lines. Loading control: Anti-COX4I1.

Applications for PICALM Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:500-1:1000

Western Blot

0.04 - 0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PICALM

Assembly protein recruiting clathrin and adaptor protein complex 2 (AP2) to cell membranes at sites of coated-pit formation and clathrin-vesicle assembly. May be required to determine the amount of membrane to be recycled, possibly by regulating the size of the clathrin cage. Involved in AP2-dependent clathrin-mediated endocytosis at the neuromuscular junction

Alternate Names

CALMclathrin assembly lymphoid-myeloid leukemia, Clathrin assembly lymphoid myeloid leukemia protein, CLTHphosphatidylinositol-binding clathrin assembly protein, LAP, phosphatidylinositol binding clathrin assembly protein

Gene Symbol

PICALM

Additional PICALM Products

Product Documents for PICALM Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for PICALM Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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