PICK1 Antibody

Novus Biologicals | Catalog # NB100-41403

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Bovine (100%), Canine (100%), Porcine (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all PICK1 Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-HYNKLQADPKQ corresponding to internal region according to NP_036539.1.

Specificity

Reported variants represent identical protein (NP_036539.1; NP_001034672.1; NP_001034673.1).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Theoretical MW

47 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PICK1 Antibody

Western Blot: PICK1 Antibody [NB100-41403]

Western Blot: PICK1 Antibody [NB100-41403]

Western Blot: PICK1 Antibody [NB100-41403] - Staining of Mouse Brain lysate (35 ug protein in RIPA buffer). Antibody at 1 ug/mL. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: PICK1 Antibody [NB100-41403]

Immunohistochemistry-Paraffin: PICK1 Antibody [NB100-41403]

Immunohistochemistry-Paraffin: PICK1 Antibody [NB100-41403] - Staining of paraffin embedded Human Prostate. Antigen at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Immunohistochemistry-Paraffin: PICK1 Antibody [NB100-41403]

Immunohistochemistry-Paraffin: PICK1 Antibody [NB100-41403]

Immunohistochemistry-Paraffin: PICK1 Antibody [NB100-41403] - Staining of paraffin embedded Human Colon. Antigen at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Applications for PICK1 Antibody

Application
Recommended Usage

Immunohistochemistry-Paraffin

5 ug/mL

Peptide ELISA

Detection limit 1:16000

Western Blot

1 - 3 ug/mL
Application Notes
WB: Approx. 50 kDa band observed in mouse brain lysates (calculated MW of 46.6 kDa band according to NP_036539.1, NP_001034672.1and NP_001034673.1).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PICK1

The protein encoded by this gene contains a PDZ domain, through which it interacts with protein kinase C, alpha (PRKCA). This protein may function as an adaptor that binds to and organizes the subcellular localization of a variety of membrane proteins. It has been shown to interact with multiple glutamate receptor subtypes, monoamine plasma membrane transporters, as well as non-voltage gated sodium channels, and may target PRKCA to these membrane proteins and thus regulate their distribution and function. This protein has also been found to act as an anchoring protein that specifically targets PRKCA to mitochondria in a ligand-specific manner. Three transcript variants encoding the same protein have been found for this gene.

Alternate Names

alpha binding protein, dJ1039K5, MGC15204, PICK, PRKCA-binding protein, PRKCABPprotein interacting with PRKCA, Protein interacting with C kinase 1, protein interacting with PRKCA 1, Protein kinase C-alpha-binding protein

Entrez Gene IDs

9463 (Human); 18693 (Mouse); 84591 (Rat)

Gene Symbol

PICK1

UniProt

Q9NRD5 (Human)

Additional PICK1 Products

Product Documents for PICK1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for PICK1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for PICK1 Antibody

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  • Q: Here is a difficult request. We are looking for an antibody with the following specifications: Anti-PICK1 Reactive in mouse without sodium azide, so it can be injected into the mouse without compromising its life With PBS (for example) It does not necessarily have to be guaranteed. Have you got anything like it?

    A: Unfortunately all of our anti-PICK1 mouse-reactive antibodies are supplied in sodium azide. We do have anti human PICK1 without preservatives, but that would not be suitable if you are working with mouse samples. NB100-41403 contains only 0.02% sodium azide which is the lowest percentage. We also offer an Antibody Concentration and Clean Up Antibody Purification Kit which can be used to reduce the concentration of unwanted additives often found in antibody formulations, such as sodium azide, glycine or tris. This kit is offered in two sizes: cat# 861-0005 includes 1 column and cat# 861-0010 includes 3 columns.

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