PIEZO1 Antibody - Azide and BSA Free
Novus Biologicals | Catalog # NBP2-10504
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Key Product Details
Validated by
Species Reactivity
Validated:
Cited:
Applications
Validated:
Cited:
Label
Antibody Source
Format
Product Specifications
Immunogen
Specificity
Clonality
Host
Isotype
Scientific Data Images for PIEZO1 Antibody - Azide and BSA Free
Western Blot: PIEZO1 Antibody [NBP2-10504] -
Western Blot: PIEZO1 Antibody [NBP2-10504] - PC2, TRPV4, & PIEZO1 localize to MLO-Y4 primary cilia & plasma membrane. (A–C) Localization of PC2, TRPV4, & PIEZO1 on MLO-Y4 primary cilia & plasma membrane. Acetylated alpha-tubulin is in enriched primary cilia. Scale bars, 10 μm. (D) Chemiluminescence-detected Western blot of PC2, TRPV4, PIEZO1, & actin protein in MLO-Y4s treated with scrambled control siRNA & Pkd2, Trpv4, or Piezo1 siRNA. (E) Quantified knockdown of Pkd2 (n = 4), Trpv4 (n = 10), & Piezo1 (n = 4) mRNA expression in MLO-Y4 cells treated with siRNA relative to controls (n = 10). Error bars show ±SEM. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/26029358), licensed under a CC-BY license. Not internally tested by Novus Biologicals.Applications for PIEZO1 Antibody - Azide and BSA Free
Immunohistochemistry
Western Blot
Formulation, Preparation, and Storage
Purification
Reconstitution
Formulation
Format
Preservative
Concentration
Shipping
Stability & Storage
Calculators
Background: PIEZO1
Long Name
Alternate Names
Gene Symbol
UniProt
Additional PIEZO1 Products
Product Documents for PIEZO1 Antibody - Azide and BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for PIEZO1 Antibody - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Citations for PIEZO1 Antibody - Azide and BSA Free
Powered by Bioz
Customer Reviews for PIEZO1 Antibody - Azide and BSA Free
There are currently no reviews for this product. Be the first to review PIEZO1 Antibody - Azide and BSA Free and earn rewards!
Have you used PIEZO1 Antibody - Azide and BSA Free?
Submit a review and receive an Amazon gift card!
$25/€18/£15/$25CAN/¥2500 for a review with an image
$10/€7/£6/$10CAN/¥1110 for a review without an image
Submit a review
Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
FAQs for PIEZO1 Antibody - Azide and BSA Free
-
Q: Can any WB or IHC images produced by NBP2-10504 be provided for references? What is the suggested dilution titer for IHC with this antibody?
A: The recommended dilution for IHC using this antibody is 1:50 to1:500. Unfortunately we do not have any images to provide at this time.
-
Q: We are currently using NBP2-10504 for IHC and we are planning to run indirect ELISA. Do you have any suggestions for recommended antibody concentrations to use for indirect ELISA?
A: The recommended dilution that you can start with your ELISA is 100ng/mL.
-
Q: Can any WB or IHC images produced by NBP2-10504 be provided for references? What is the suggested dilution titer for IHC with this antibody?
A: The recommended dilution for IHC using this antibody is 1:50 to1:500. Unfortunately we do not have any images to provide at this time.
-
Q: We are currently using NBP2-10504 for IHC and we are planning to run indirect ELISA. Do you have any suggestions for recommended antibody concentrations to use for indirect ELISA?
A: The recommended dilution that you can start with your ELISA is 100ng/mL.