PIEZO1 Antibody - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-10504

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse, Rat, Porcine, Rabbit

Cited:

Mouse, Rat

Applications

Validated:

Immunohistochemistry, Western Blot, ELISA, Knockdown Validated

Cited:

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free
View all PIEZO1 Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide derived from C-terminal domain of human Protein FAM38A.

Specificity

Reacts with human 287 kDa Protein FAM38A. Cross-reacts with mouse, rat, rabbit and other species due to sequence homology. Piezo-type mechanosensitive ion channel component 1.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PIEZO1 Antibody - Azide and BSA Free

PIEZO1 Antibody

Western Blot: PIEZO1 Antibody [NBP2-10504] -

Western Blot: PIEZO1 Antibody [NBP2-10504] - PC2, TRPV4, & PIEZO1 localize to MLO-Y4 primary cilia & plasma membrane. (A–C) Localization of PC2, TRPV4, & PIEZO1 on MLO-Y4 primary cilia & plasma membrane. Acetylated alpha-tubulin is in enriched primary cilia. Scale bars, 10 μm. (D) Chemiluminescence-detected Western blot of PC2, TRPV4, PIEZO1, & actin protein in MLO-Y4s treated with scrambled control siRNA & Pkd2, Trpv4, or Piezo1 siRNA. (E) Quantified knockdown of Pkd2 (n = 4), Trpv4 (n = 10), & Piezo1 (n = 4) mRNA expression in MLO-Y4 cells treated with siRNA relative to controls (n = 10). Error bars show ±SEM. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/26029358), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for PIEZO1 Antibody - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

1:50-1:500

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Lyophilized powder, reconstitute to 1 mg/ml in distilled water.

Formulation

0.1M Tris, 0.1M Glycine and 2% Sucrose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: PIEZO1

PIEZO1 (piezo-type mechanosensitive ion channel component 1) plays a crucial role in epithelial cell adhesion. PIEZO1 belongs to the PIEZO family of transmembrane proteins. Mutations in Piezo1 have been tied to Xerocytosis. PIEZO1 has been studied in relation to neuronitis, Alzheimer's disease and Parkinson's disease.

Long Name

Piezo-type mechanosensitive ion channel component 1

Alternate Names

FAM38A, KIAA0233, Mib, Protein FAM38A

Entrez Gene IDs

9780 (Human); 234839 (Mouse); 361430 (Rat)

Gene Symbol

PIEZO1

UniProt

Q92508 (Human)

Additional PIEZO1 Products

Product Documents for PIEZO1 Antibody - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for PIEZO1 Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for PIEZO1 Antibody - Azide and BSA Free

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for PIEZO1 Antibody - Azide and BSA Free

Showing  1 - 2 of 2 FAQs Showing All

  • Q: Can any WB or IHC images produced by NBP2-10504 be provided for references? What is the suggested dilution titer for IHC with this antibody?

    A: The recommended dilution for IHC using this antibody is 1:50 to1:500. Unfortunately we do not have any images to provide at this time.

  • Q: We are currently using NBP2-10504 for IHC and we are planning to run indirect ELISA. Do you have any suggestions for recommended antibody concentrations to use for indirect ELISA?

    A: The recommended dilution that you can start with your ELISA is 100ng/mL.

  • Q: Can any WB or IHC images produced by NBP2-10504 be provided for references? What is the suggested dilution titer for IHC with this antibody?

    A: The recommended dilution for IHC using this antibody is 1:50 to1:500. Unfortunately we do not have any images to provide at this time.

  • Q: We are currently using NBP2-10504 for IHC and we are planning to run indirect ELISA. Do you have any suggestions for recommended antibody concentrations to use for indirect ELISA?

    A: The recommended dilution that you can start with your ELISA is 100ng/mL.

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