PIG3 Antibody (1C9) - BSA Free

Novus Biologicals | Catalog # NBP2-22591

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 1C9

Format

BSA Free
View all PIG3 Primary Antibodies »

Product Specifications

Immunogen

Recombinant human PIG3 (1-332aa) purified from E. coli

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for PIG3 Antibody (1C9) - BSA Free

Western Blot: PIG3 Antibody (1C9)BSA Free [NBP2-22591]

Western Blot: PIG3 Antibody (1C9)BSA Free [NBP2-22591]

Western Blot: PIG3 Antibody (1C9) [NBP2-22591] - The Cell lysates (1ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PIG3 antibody (1:3000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: 293T cell lysate Lane 2.: PIG3 Transfected 293T cell lysate
Immunocytochemistry/ Immunofluorescence: PIG3 Antibody (1C9) - BSA Free [NBP2-22591]

Immunocytochemistry/ Immunofluorescence: PIG3 Antibody (1C9) - BSA Free [NBP2-22591]

Immunocytochemistry/Immunofluorescence: PIG3 Antibody (1C9) [NBP2-22591] - ICC/IF analysis of PIG3 in A549 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human PIG3 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).
Flow Cytometry: PIG3 Antibody (1C9) - BSA Free [NBP2-22591]

Flow Cytometry: PIG3 Antibody (1C9) - BSA Free [NBP2-22591]

Flow Cytometry: PIG3 Antibody (1C9) [NBP2-22591] - Flow cytometry analysis of PIG3 in A549 cell line, staining at 2-5ug for 1x106cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).
Western Blot: PIG3 Antibody (1C9)BSA Free [NBP2-22591]

Western Blot: PIG3 Antibody (1C9)BSA Free [NBP2-22591]

Western Blot: PIG3 Antibody (1C9) [NBP2-22591] - The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PIG3 antibody (1:3000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1. : A549 cell lysate Lane 2. : SW480 cell lysate

Applications for PIG3 Antibody (1C9) - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:3000

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.4), 10% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PIG3

TP53I3 participates in the generation of reactive oxygen species (ROS). TP53I3 has low NADPH-dependent naphtoquinone reductase activity, with a preference for 1,2-naphtoquinone over 1,4-naphtoquinone. TP53I3 has low NADPH-dependent diamine reductase activity (in vitro). TP53I3 is localized to the cytoplasm and induced in primary, non-transformed and transformed cell cultures after exposure to genotoxic agents. TP53I3 microsatellite polymorphism is associated with differential susceptibility to cancer.

Alternate Names

p53-induced gene 3 protein, PIG3quinone oxidoreductase homolog, quinone oxidoreductase PIG3, tumor protein p53 inducible protein 3, Tumor protein p53-inducible protein 3

Gene Symbol

TP53I3

UniProt

NP_671713 (Human)

Additional PIG3 Products

Product Documents for PIG3 Antibody (1C9) - BSA Free

Certificate of Analysis

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Product Specific Notices for PIG3 Antibody (1C9) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for PIG3 Antibody (1C9) - BSA Free

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Protocols

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