PIK3R4 Antibody

Novus Biologicals | Catalog # NBP1-30463

Novus Biologicals
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Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat, Zebrafish

Cited:

Human, Mouse, Rat, Fish - Danio rerio (Zebrafish)

Predicted:

Orangutan (100%). Backed by our 100% Guarantee.

Applications

Validated:

Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Cited:

Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all PIK3R4 Primary Antibodies »

Product Specifications

Immunogen

The immunogen for this product maps to a region between residue 1300 and 1358 of human vacuolar protein sorting 15 using the numbering given in entry NP_055417.1 (GeneID 30849).

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 25961502). Zebrafish reactivity reported in scientific literature (PMID: 27882921).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

153 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PIK3R4 Antibody

Western Blot: PIK3R4 Antibody [NBP1-30463]

Western Blot: PIK3R4 Antibody [NBP1-30463]

Western Blot: PIK3R4 Antibody [NBP1-30463] - Whole cell lysate from HeLa, 293T and mouse NIH3T3 cells. Vps15 was also immunoprecipitated by rabbit anti-Vps15 antibody NBP1-30462.

Applications for PIK3R4 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

Reported in scientific literature (PMID 24089209)

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:2000-1:10000
Application Notes
Western blot of lysates performed using standard western blot reagents and 4-8% SDS-PAGE.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: PIK3R4

Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains.

Long Name

Phosphoinositide-3-kinase, Regulatory Subunit 4

Alternate Names

p150, VPS15

Entrez Gene IDs

30849 (Human); 75669 (Mouse)

Gene Symbol

PIK3R4

UniProt

Q99570

Additional PIK3R4 Products

Product Documents for PIK3R4 Antibody

Certificate of Analysis

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Download SDS

Product Specific Notices for PIK3R4 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for PIK3R4 Antibody

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Protocols

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FAQs

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Associated Pathways

Akt Signaling Pathway Akt Signaling Pathway Thumbnail