Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # OTI5D5
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Product Specifications
Immunogen
Full length human recombinant protein of human PIM2 (NP_006866) produced in HEK293T cell.
Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Theoretical MW
34 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Novus Biologicals Mouse PIM2 Antibody (OTI5D5) (NBP2-02441) is a monoclonal antibody validated for use in IHC, WB, Flow, ICC/IF and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for PIM2 Antibody (OTI5D5)
![Western Blot: PIM2 Antibody (OTI5D5) [NBP2-02441]](https://beta-resources.rndsystems.com/images/products/PIM2-Antibody-OTI5D5-Western-Blot-NBP2-02441-img0003.jpg "Western Blot: PIM2 Antibody (OTI5D5) [NBP2-02441]")
Western Blot: PIM2 Antibody (OTI5D5) [NBP2-02441]
Western Blot: PIM2 Antibody (OTI5D5) [NBP2-02441] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PIM2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PIM2.![Immunocytochemistry/ Immunofluorescence: PIM2 Antibody (OTI5D5) [NBP2-02441]](https://beta-resources.rndsystems.com/images/products/PIM2-Antibody-OTI5D5-Immunocytochemistry-Immunofluorescence-NBP2-02441-img0011.jpg "Immunocytochemistry/ Immunofluorescence: PIM2 Antibody (OTI5D5) [NBP2-02441]")
Immunocytochemistry/ Immunofluorescence: PIM2 Antibody (OTI5D5) [NBP2-02441]
Immunocytochemistry/Immunofluorescence: PIM2 Antibody (OTI5D5) [NBP2-02441] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY PIM2.![Immunohistochemistry-Paraffin: PIM2 Antibody (OTI5D5) [NBP2-02441]](https://beta-resources.rndsystems.com/images/products/PIM2-Antibody-OTI5D5-Immunohistochemistry-Paraffin-NBP2-02441-img0010.jpg "Immunohistochemistry-Paraffin: PIM2 Antibody (OTI5D5) [NBP2-02441]")
Immunohistochemistry-Paraffin: PIM2 Antibody (OTI5D5) [NBP2-02441]
Immunohistochemistry-Paraffin: PIM2 Antibody (OTI5D5) [NBP2-02441] - Staining of paraffin-embedded Human prostate tissue using anti-PIM2 mouse monoclonal antibody.![Flow Cytometry: PIM2 Antibody (OTI5D5) [NBP2-02441]](https://beta-resources.rndsystems.com/images/products/PIM2-Antibody-OTI5D5-Flow-Cytometry-NBP2-02441-img0002.jpg "Flow Cytometry: PIM2 Antibody (OTI5D5) [NBP2-02441]")
Flow Cytometry: PIM2 Antibody (OTI5D5) [NBP2-02441]
Flow Cytometry: PIM2 Antibody (OTI5D5) [NBP2-02441] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PIM2 antibody, and then analyzed by flow cytometry.![Immunocytochemistry/ Immunofluorescence: PIM2 Antibody (OTI5D5) [NBP2-02441]](https://beta-resources.rndsystems.com/images/products/PIM2-Antibody-OTI5D5-Immunocytochemistry-Immunofluorescence-NBP2-02441-img0004.jpg "Immunocytochemistry/ Immunofluorescence: PIM2 Antibody (OTI5D5) [NBP2-02441]")
Immunocytochemistry/ Immunofluorescence: PIM2 Antibody (OTI5D5) [NBP2-02441]
Immunocytochemistry/Immunofluorescence: PIM2 Antibody (OTI5D5) [NBP2-02441] - Immunofluorescent staining of HeLa cells using anti-PIM2 mouse monoclonal antibody.![Immunocytochemistry/ Immunofluorescence: PIM2 Antibody (OTI5D5) [NBP2-02441]](https://beta-resources.rndsystems.com/images/products/PIM2-Antibody-OTI5D5-Immunocytochemistry-Immunofluorescence-NBP2-02441-img0005.jpg "Immunocytochemistry/ Immunofluorescence: PIM2 Antibody (OTI5D5) [NBP2-02441]")
Immunocytochemistry/ Immunofluorescence: PIM2 Antibody (OTI5D5) [NBP2-02441]
Immunocytochemistry/Immunofluorescence: PIM2 Antibody (OTI5D5) [NBP2-02441] - Immunofluorescent staining of HT29 cells using anti-PIM2 mouse monoclonal antibody.![Immunohistochemistry-Paraffin: PIM2 Antibody (OTI5D5) [NBP2-02441]](https://beta-resources.rndsystems.com/images/products/PIM2-Antibody-OTI5D5-Immunohistochemistry-Paraffin-NBP2-02441-img0006.jpg "Immunohistochemistry-Paraffin: PIM2 Antibody (OTI5D5) [NBP2-02441]")
Immunohistochemistry-Paraffin: PIM2 Antibody (OTI5D5) [NBP2-02441]
Immunohistochemistry-Paraffin: PIM2 Antibody (OTI5D5) [NBP2-02441] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-PIM2 mouse monoclonal antibody.![Immunohistochemistry-Paraffin: PIM2 Antibody (OTI5D5) [NBP2-02441]](https://beta-resources.rndsystems.com/images/products/PIM2-Antibody-OTI5D5-Immunohistochemistry-Paraffin-NBP2-02441-img0007.jpg "Immunohistochemistry-Paraffin: PIM2 Antibody (OTI5D5) [NBP2-02441]")
Immunohistochemistry-Paraffin: PIM2 Antibody (OTI5D5) [NBP2-02441]
Immunohistochemistry-Paraffin: PIM2 Antibody (OTI5D5) [NBP2-02441] - Staining of paraffin-embedded Human Kidney tissue using anti-PIM2 mouse monoclonal antibody.![Immunohistochemistry-Paraffin: PIM2 Antibody (OTI5D5) [NBP2-02441]](https://beta-resources.rndsystems.com/images/products/PIM2-Antibody-OTI5D5-Immunohistochemistry-Paraffin-NBP2-02441-img0008.jpg "Immunohistochemistry-Paraffin: PIM2 Antibody (OTI5D5) [NBP2-02441]")
Immunohistochemistry-Paraffin: PIM2 Antibody (OTI5D5) [NBP2-02441]
Immunohistochemistry-Paraffin: PIM2 Antibody (OTI5D5) [NBP2-02441] - Staining of paraffin-embedded Human liver tissue using anti-PIM2 mouse monoclonal antibody.![Immunohistochemistry-Paraffin: PIM2 Antibody (OTI5D5) [NBP2-02441]](https://beta-resources.rndsystems.com/images/products/PIM2-Antibody-OTI5D5-Immunohistochemistry-Paraffin-NBP2-02441-img0009.jpg "Immunohistochemistry-Paraffin: PIM2 Antibody (OTI5D5) [NBP2-02441]")
Immunohistochemistry-Paraffin: PIM2 Antibody (OTI5D5) [NBP2-02441]
Immunohistochemistry-Paraffin: PIM2 Antibody (OTI5D5) [NBP2-02441] - Staining of paraffin-embedded Human pancreas tissue using anti-PIM2 mouse monoclonal antibody.![Flow Cytometry: PIM2 Antibody (OTI5D5) [NBP2-02441]](https://beta-resources.rndsystems.com/images/products/PIM2-Antibody-OTI5D5-Flow-Cytometry-NBP2-02441-img0001.jpg "Flow Cytometry: PIM2 Antibody (OTI5D5) [NBP2-02441]")
Flow Cytometry: PIM2 Antibody (OTI5D5) [NBP2-02441]
Flow Cytometry: PIM2 Antibody (OTI5D5) [NBP2-02441] - Analysis of Jurkat cells, using anti-PIM2 antibody, (Red), compared to a nonspecific negative control antibody (Blue).![Immunoprecipitation: PIM2 Antibody (OTI5D5) [NBP2-02441]](https://beta-resources.rndsystems.com/images/products/PIM2-Antibody-OTI5D5-Immunoprecipitation-NBP2-02441-img0012.jpg "Immunoprecipitation: PIM2 Antibody (OTI5D5) [NBP2-02441]")
Immunoprecipitation: PIM2 Antibody (OTI5D5) [NBP2-02441]
Immunoprecipitation: PIM2 Antibody (OTI5D5) [NBP2-02441] - (Negative control: IP without adding anti-PIM2 antibody.). For each experiment, 500ul of DDK tagged PIM2 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-PIM2 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.Applications for PIM2 Antibody (OTI5D5)
Application
Recommended Usage
Flow Cytometry
1:100
Immunocytochemistry/ Immunofluorescence
1:100
Immunohistochemistry
1:50
Immunohistochemistry-Paraffin
1:50
Immunoprecipitation
2ug/500ul
Western Blot
1:2000
Flow Cytometry Panel Builder
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Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
0.54 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: PIM2
Long Name
Proviral Integration Site 2
Alternate Names
EC 2.7.11.1, pim-2 oncogene, Pim-2h, proto-oncogene Pim-2 (serine threonine kinase), serine/threonine protein kinase pim-2, serine/threonine-protein kinase pim-2
Entrez Gene IDs
11040 (Human)
Gene Symbol
PIM2
Additional PIM2 Products
Product Documents for PIM2 Antibody (OTI5D5)
Product Specific Notices for PIM2 Antibody (OTI5D5)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- 7-Amino Actinomycin D (7-AAD) Cell Viability Flow Cytometry Protocol
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- Detection & Visualization of Antibody Binding
- Extracellular Membrane Flow Cytometry Protocol
- Flow Cytometry Protocol for Cell Surface Markers
- Flow Cytometry Protocol for Staining Membrane Associated Proteins
- Flow Cytometry Staining Protocols
- Flow Cytometry Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Immunoprecipitation Protocol
- Intracellular Flow Cytometry Protocol Using Alcohol (Methanol)
- Intracellular Flow Cytometry Protocol Using Detergents
- Intracellular Nuclear Staining Flow Cytometry Protocol Using Detergents
- Intracellular Staining Flow Cytometry Protocol Using Alcohol Permeabilization
- Intracellular Staining Flow Cytometry Protocol Using Detergents to Permeabilize Cells
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Propidium Iodide Cell Viability Flow Cytometry Protocol
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Characterization of Human Th22 Cells
- Protocol for the Characterization of Human Th9 Cells
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
FAQs for PIM2 Antibody (OTI5D5)
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Q: We would like to work with one of your 6 Pim 2 antibodies suitable for flow cytometry. We would like to use it for intracellular staining by FACS analysis. Now we saw that "There are no reviews for PIM2 Antibody (NBP2-02448)(NBP2-02441)(NBP2-02536)(NBP2-02439)(NBP2-02440)(NBP2-02442)." As there are no refferences I would like to know if you could send us a test sample. And please, as there are 6 Pim2 FLOW antibodys, which one would you recomend for us?
A: I recommend NBP2-02536 or NBP2-02441 for Pim2 detection as these have been the most extensively validated in flow cytometry.
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