PIM2 Antibody (OTI5D5) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-73412

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # OTI5D5

Format

Azide and BSA Free
View all PIM2 Primary Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human PIM2 (NP_006866) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

34 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Mouse PIM2 Antibody (OTI5D5) - Azide and BSA Free (NBP2-02441) is a monoclonal antibody validated for use in IHC, WB, Flow, ICC/IF and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for PIM2 Antibody (OTI5D5) - Azide and BSA Free

Western Blot: PIM2 Antibody (OTI5D5)Azide and BSA Free [NBP2-73412]

Western Blot: PIM2 Antibody (OTI5D5)Azide and BSA Free [NBP2-73412]

Western Blot: PIM2 Antibody (OTI5D5) - Azide and BSA Free [NBP2-73412] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PIM2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PIM2.
Immunocytochemistry/ Immunofluorescence: PIM2 Antibody (OTI5D5) - Azide and BSA Free [NBP2-73412]

Immunocytochemistry/ Immunofluorescence: PIM2 Antibody (OTI5D5) - Azide and BSA Free [NBP2-73412]

Immunocytochemistry/Immunofluorescence: PIM2 Antibody (OTI5D5) - Azide and BSA Free [NBP2-73412] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY PIM2.
Immunohistochemistry: PIM2 Antibody (OTI5D5) - Azide and BSA Free [NBP2-73412]

Immunohistochemistry: PIM2 Antibody (OTI5D5) - Azide and BSA Free [NBP2-73412]

Immunohistochemistry: PIM2 Antibody (OTI5D5) - Azide and BSA Free [NBP2-73412] - Staining of paraffin-embedded Human prostate tissue using anti-PIM2 mouse monoclonal antibody.
Flow Cytometry: PIM2 Antibody (OTI5D5) - Azide and BSA Free [NBP2-73412]

Flow Cytometry: PIM2 Antibody (OTI5D5) - Azide and BSA Free [NBP2-73412]

Flow Cytometry: PIM2 Antibody (OTI5D5) - Azide and BSA Free [NBP2-73412] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-PIM2 antibody, and then analyzed by flow cytometry.
Immunocytochemistry/ Immunofluorescence: PIM2 Antibody (OTI5D5) - Azide and BSA Free [NBP2-73412]

Immunocytochemistry/ Immunofluorescence: PIM2 Antibody (OTI5D5) - Azide and BSA Free [NBP2-73412]

Immunocytochemistry/Immunofluorescence: PIM2 Antibody (OTI5D5) - Azide and BSA Free [NBP2-73412] - Staining of HeLa cells using anti-PIM2 mouse monoclonal antibody.
Immunocytochemistry/ Immunofluorescence: PIM2 Antibody (OTI5D5) - Azide and BSA Free [NBP2-73412]

Immunocytochemistry/ Immunofluorescence: PIM2 Antibody (OTI5D5) - Azide and BSA Free [NBP2-73412]

Immunocytochemistry/Immunofluorescence: PIM2 Antibody (OTI5D5) - Azide and BSA Free [NBP2-73412] - Staining of HT29 cells using anti-PIM2 mouse monoclonal antibody.
Immunohistochemistry: PIM2 Antibody (OTI5D5) - Azide and BSA Free [NBP2-73412]

Immunohistochemistry: PIM2 Antibody (OTI5D5) - Azide and BSA Free [NBP2-73412]

Immunohistochemistry: PIM2 Antibody (OTI5D5) - Azide and BSA Free [NBP2-73412] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-PIM2 mouse monoclonal antibody.
Immunohistochemistry: PIM2 Antibody (OTI5D5) - Azide and BSA Free [NBP2-73412]

Immunohistochemistry: PIM2 Antibody (OTI5D5) - Azide and BSA Free [NBP2-73412]

Immunohistochemistry: PIM2 Antibody (OTI5D5) - Azide and BSA Free [NBP2-73412] - Staining of paraffin-embedded Human Kidney tissue using anti-PIM2 mouse monoclonal antibody.
Immunohistochemistry: PIM2 Antibody (OTI5D5) - Azide and BSA Free [NBP2-73412]

Immunohistochemistry: PIM2 Antibody (OTI5D5) - Azide and BSA Free [NBP2-73412]

Immunohistochemistry: PIM2 Antibody (OTI5D5) - Azide and BSA Free [NBP2-73412] - Staining of paraffin-embedded Human liver tissue using anti-PIM2 mouse monoclonal antibody.
Immunohistochemistry: PIM2 Antibody (OTI5D5) - Azide and BSA Free [NBP2-73412]

Immunohistochemistry: PIM2 Antibody (OTI5D5) - Azide and BSA Free [NBP2-73412]

Immunohistochemistry: PIM2 Antibody (OTI5D5) - Azide and BSA Free [NBP2-73412] - Staining of paraffin-embedded Human pancreas tissue using anti-PIM2 mouse monoclonal antibody.
Flow Cytometry: PIM2 Antibody (OTI5D5) - Azide and BSA Free [NBP2-73412]

Flow Cytometry: PIM2 Antibody (OTI5D5) - Azide and BSA Free [NBP2-73412]

Flow Cytometry: PIM2 Antibody (OTI5D5) - Azide and BSA Free [NBP2-73412] - Analysis of Jurkat cells, using anti-PIM2 antibody, (Red), compared to a nonspecific negative control antibody (Blue).
Immunoprecipitation: PIM2 Antibody (OTI5D5) - Azide and BSA Free [NBP2-73412]

Immunoprecipitation: PIM2 Antibody (OTI5D5) - Azide and BSA Free [NBP2-73412]

Immunoprecipitation: PIM2 Antibody (OTI5D5) - Azide and BSA Free [NBP2-73412] - (Negative control: IP without adding anti-PIM2 antibody.). For each experiment, 500ul of DDK tagged PIM2 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-PIM2 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.

Applications for PIM2 Antibody (OTI5D5) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:50

Immunoprecipitation

2ug/500ul

Western Blot

1:2000

Flow Cytometry Panel Builder

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: PIM2

PIM2 belongs to a family of cytoplasmic serine/threonine protein kinases that consists of two other members, PIM1 and PIM3. Expression of this protein is regulated by hematopoietic cytokines. PIM2 is most abundantly expressed in hematopoietic tissues, spleen, thymus, and peripheral blood leukocytes, as well as in testis, small intestine, and colon. It is also highly expressed in human leukemic and lymphoma cell lines. The gene is also expressed in primary spermatocytes. The results suggest a role for PIM2 in proliferating cells as well as during meiosis. PIM2 is thought to be a mediator of p53 mediated apoptosis inhibition by interleukin 3, which supports its role in the formation of various tumours including lymphomas.

Long Name

Proviral Integration Site 2

Alternate Names

EC 2.7.11.1, pim-2 oncogene, Pim-2h, proto-oncogene Pim-2 (serine threonine kinase), serine/threonine protein kinase pim-2, serine/threonine-protein kinase pim-2

Gene Symbol

PIM2

Additional PIM2 Products

Product Documents for PIM2 Antibody (OTI5D5) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for PIM2 Antibody (OTI5D5) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Epigenetics

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Protocols

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FAQs

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