Pirin Antibody

Novus Biologicals | Catalog # NB300-958

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Pirin Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence GSSKKVTLSVLSREC corresponding to N-Terminus according to NP_003653.1.

Specificity

Variants (NP_003653.1; NP_001018119.1) encode the same protein.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Pirin Antibody

Western Blot: Pirin Antibody [NB300-958]

Western Blot: Pirin Antibody [NB300-958]

Western Blot: Pirin Antibody [NB300-958] - Staining of Human Heart lysate (35 ug protein in RIPA buffer). Antibody at 0.3 ug/mL. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: Pirin Antibody [NB300-958]

Immunohistochemistry-Paraffin: Pirin Antibody [NB300-958]

Immunohistochemistry-Paraffin: Pirin Antibody [NB300-958] - Staining of paraffin embedded Human Spleen. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Immunohistochemistry-Paraffin: Pirin Antibody [NB300-958]

Immunohistochemistry-Paraffin: Pirin Antibody [NB300-958]

Immunohistochemistry-Paraffin: Pirin Antibody [NB300-958] - Staining of paraffin embedded Human Liver. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Applications for Pirin Antibody

Application
Recommended Usage

Immunohistochemistry-Paraffin

5 ug/mL

Peptide ELISA

Detection limit 1:128000

Western Blot

0.3 - 1.0 ug/mL
Application Notes
WB: Approx. 33 kDa band observed in human heart lysate (calculated MW of 32.1 kDa band according to NP_003653.1).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Pirin

This gene encodes a member of the cupin superfamily. The encoded protein is an Fe(II)-containing nuclear protein expressed in all tissues of the body and concentrated within dot-like subnuclear structures. Interactions with nuclear factor I/CCAAT box transcription factor as well as B cell lymphoma 3-encoded oncoprotein suggest the encoded protein may act as a transcriptional cofactor and be involved in the regulation of DNA transcription and replication. Alternatively spliced transcript variants have been described.

Alternate Names

EC 1.13.11.24, pirin, pirin (iron-binding nuclear protein), Probable quercetin 2,3-dioxygenase PIR, Probable quercetinase

Entrez Gene IDs

8544 (Human); 69656 (Mouse)

Gene Symbol

PIR

UniProt

NP_003653. (Human)

Additional Pirin Products

Product Documents for Pirin Antibody

Certificate of Analysis

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Product Specific Notices for Pirin Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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