PKN2 Antibody (1D1)

Novus Biologicals | Catalog # NBP2-37573

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat, Monkey, Primate

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 1D1
View all PKN2 Primary Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human PKN2 expressed in E. Coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

140 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PKN2 Antibody (1D1)

Western Blot: PKN2 Antibody (1D1)BSA Free [NBP2-37573]

Western Blot: PKN2 Antibody (1D1)BSA Free [NBP2-37573]

Western Blot: PKN2 Antibody (1D1) [NBP2-37573] - Western blot analysis using PRK2 mouse mAb against PC-12 (1), Cos7 (2), K562 (3), Jurkat (4), Hela (5), A431 (6), C6 (7), NIH/3T3 (8) and HEK293 (9) cell lysate.
Immunohistochemistry-Paraffin: PKN2 Antibody (1D1) - BSA Free [NBP2-37573]

Immunohistochemistry-Paraffin: PKN2 Antibody (1D1) - BSA Free [NBP2-37573]

Immunohistochemistry-Paraffin: PKN2 Antibody (1D1) [NBP2-37573] - Analysis of lung cancer tissues using PRK2 mouse mAb with DAB staining.
Flow Cytometry: PKN2 Antibody (1D1) - BSA Free [NBP2-37573]

Flow Cytometry: PKN2 Antibody (1D1) - BSA Free [NBP2-37573]

Flow Cytometry: PKN2 Antibody (1D1) [NBP2-37573] - Flow cytometric analysis of NIH/3T3 cells using PRK2 mouse mAb (blue) and negative control (red).
Western Blot: PKN2 Antibody (1D1)BSA Free [NBP2-37573]

Western Blot: PKN2 Antibody (1D1)BSA Free [NBP2-37573]

Western Blot: PKN2 Antibody (1D1) [NBP2-37573] - Western blot analysis using PRK2 mAb against human PRK2 (AA: 555-718) recombinant protein. (Expected MW is 43.9 kDa)
Immunohistochemistry-Paraffin: PKN2 Antibody (1D1) - BSA Free [NBP2-37573]

Immunohistochemistry-Paraffin: PKN2 Antibody (1D1) - BSA Free [NBP2-37573]

Immunohistochemistry-Paraffin: PKN2 Antibody (1D1) [NBP2-37573] - Analysis of prostate tissues using PRK2 mouse mAb with DAB staining.
ELISA: PKN2 Antibody (1D1) - BSA Free [NBP2-37573]

ELISA: PKN2 Antibody (1D1) - BSA Free [NBP2-37573]

ELISA: PKN2 Antibody (1D1) [NBP2-37573] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Applications for PKN2 Antibody (1D1)

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200 - 1:400

Immunohistochemistry

1:200 - 1:1000

Western Blot

1:500 - 1:2000

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Ascites

Formulation

Ascites

Preservative

0.03% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PKN2

Protein-kinase-C-related kinases (PRKs) (1) are part of the lipid-regulated protein kinases (PKC) which also include liver PAK & PKN (2-3). Human PRK1 and PRK2 share structurally similar catalytic domains, but less similar N-terminal regulatory regions suggesting different regulatory domain functions. PRK1 and PRK2, as well as a third member of this family, PRK3, show distinct patterns of expression in adult tissues. Additionally, the serine-threonine kinase PRK2 can be specifically cleaved by caspase-3 (and/or caspase-3-like subfamily members) during apoptosis (4).

Long Name

Protein Kinase N2

Alternate Names

PAK2, PRK2, PRKCL2, PRO2042

Gene Symbol

PKN2

Additional PKN2 Products

Product Documents for PKN2 Antibody (1D1)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for PKN2 Antibody (1D1)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Diabetes
Intracellular Kinases

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Protocols

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FAQs

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