PLA2G4A Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-31344

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Predicted:

Bovine (92%), Rat (95%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all PLA2G4A Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human PLA2G4A. The exact sequence is proprietary.

Localization

Cytoplasm, Cytoplasmic vesicle

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

85 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PLA2G4A Antibody - BSA Free

Western Blot: PLA2G4A Antibody [NBP1-31344]

Western Blot: PLA2G4A Antibody [NBP1-31344]

Western Blot: PLA2G4A Antibody [NBP1-31344] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PLA2G4A antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunocytochemistry/ Immunofluorescence: PLA2G4A Antibody [NBP1-31344]

Immunocytochemistry/ Immunofluorescence: PLA2G4A Antibody [NBP1-31344]

Immunocytochemistry/Immunofluorescence: PLA2G4A Antibody [NBP1-31344] - Analysis of HeLa, using NBP1-31344 at 1:200 dilution.
Immunohistochemistry-Paraffin: PLA2G4A Antibody [NBP1-31344]

Immunohistochemistry-Paraffin: PLA2G4A Antibody [NBP1-31344]

Immunohistochemistry-Paraffin: PLA2G4A Antibody [NBP1-31344] - PLA2G4A antibody detects PLA2G4A protein at cytoplasm in mouse liver by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. PLA2G4A antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Western Blot: PLA2G4A Antibody [NBP1-31344]

Western Blot: PLA2G4A Antibody [NBP1-31344]

Western Blot: PLA2G4A Antibody [NBP1-31344] - Sample (30 ug of whole cell lysate) A: NIH-3T3 7.5% SDS PAGE; antibody diluted at 1:1000.
Immunohistochemistry-Paraffin: PLA2G4A Antibody [NBP1-31344]

Immunohistochemistry-Paraffin: PLA2G4A Antibody [NBP1-31344]

Immunohistochemistry-Paraffin: PLA2G4A Antibody [NBP1-31344] - U87 xenograft, using PLA2G4A antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Immunohistochemistry-Paraffin: PLA2G4A Antibody [NBP1-31344]

Immunohistochemistry-Paraffin: PLA2G4A Antibody [NBP1-31344]

Immunohistochemistry-Paraffin: PLA2G4A Antibody [NBP1-31344] - PLA2G4A antibody detects PLA2G4A protein at cytoplasm in mouse duodenum by immunohistochemical analysis. Sample: Paraffin-embedded mouse duodenum.PLA2G4A antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Applications for PLA2G4A Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1% BSA, 20% Glycerol

Format

BSA Free

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: PLA2G4A

This gene encodes a member of the cytosolic phospholipase A2 group IV family. The enzyme catalyzes the hydrolysis of membrane phospholipids to release arachidonic acid which is subsequently metabolized into eicosanoids. Eicosanoids, including prostaglandins and leukotrienes, are lipid-based cellular hormones that regulate hemodynamics, inflammatory responses, and other intracellular pathways. The hydrolysis reaction also produces lysophospholipids that are converted into platelet-activating factor. The enzyme is activated by increased intracellular Ca(2+) levels and phosphorylation, resulting in its translocation from the cytosol and nucleus to perinuclear membrane vesicles.

Long Name

Phospholipase A2 Group IV A

Alternate Names

cPLA2, cPLA2-alpha

Entrez Gene IDs

5321 (Human); 18783 (Mouse)

Gene Symbol

PLA2G4A

UniProt

P47712 (Human)

Additional PLA2G4A Products

Product Documents for PLA2G4A Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for PLA2G4A Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for PLA2G4A Antibody - BSA Free

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  • Q: Hi. Actually I want to purchase anti cPLA2 antibody raised in rabbit which can bind to both phorphorylated and unphosphorylated mouse cPLA2 group IV. I want to a gel shift assay with western blotting to show that my protein of interest is causing the phosphorylation of cPLA2 inside the mouse macrophages RAW cells.Can you please tell me which antibody would be suitable for the purpose. Thanks a lot.

    A: Here are our antibodies raised in rabbit that have been validated to detect cPLA2 in mouse samples in a western blot. We have not specifically confirmed the ability of these antibodies to detect both the phosphorylated and unphosphorylated forms of the protein, and I cannot guarantee that they will clearly differentiate between the two.

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Associated Pathways

VEGF - VEGF R2 Signaling Pathways VEGF - VEGF R2 Signaling Pathway Thumbnail