PLA2R1 Antibody - BSA Free
Novus Biologicals | Catalog # NBP1-84449
![Immunohistochemistry-Paraffin: PLA2R1 Antibody [NBP1-84449]](https://resources.rndsystems.com/images/products/PLA2R1-Antibody-Immunohistochemistry-Paraffin-NBP1-84449-img0010.jpg "Immunohistochemistry-Paraffin: PLA2R1 Antibody [NBP1-84449]")
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Scientific Data Images for PLA2R1 Antibody - BSA Free
![Immunohistochemistry-Paraffin: PLA2R1 Antibody [NBP1-84449]](https://resources.rndsystems.com/images/products/PLA2R1-Antibody-Immunohistochemistry-Paraffin-NBP1-84449-img0012.jpg "Immunohistochemistry-Paraffin: PLA2R1 Antibody [NBP1-84449]")
Immunohistochemistry-Paraffin: PLA2R1 Antibody [NBP1-84449]
Immunohistochemistry-Paraffin: PLA2R1 Antibody [NBP1-84449] - Staining o human kidney (idiopathic membranous nephropathy) shows strong membranous positivity in cells in glomeruli.![Immunohistochemistry-Paraffin: PLA2R1 Antibody [NBP1-84449]](https://resources.rndsystems.com/images/products/PLA2R1-Antibody-Immunohistochemistry-Paraffin-NBP1-84449-img0004.jpg "Immunohistochemistry-Paraffin: PLA2R1 Antibody [NBP1-84449]")
Immunohistochemistry-Paraffin: PLA2R1 Antibody [NBP1-84449]
Immunohistochemistry-Paraffin: PLA2R1 Antibody [NBP1-84449] - Immunohistochemical staining of human pancreas shows no positivity in exocrine glandular cells as expected.![Immunohistochemistry-Paraffin: PLA2R1 Antibody [NBP1-84449]](https://resources.rndsystems.com/images/products/PLA2R1-Antibody-Immunohistochemistry-Paraffin-NBP1-84449-img0008.jpg "Immunohistochemistry-Paraffin: PLA2R1 Antibody [NBP1-84449]")
Immunohistochemistry-Paraffin: PLA2R1 Antibody [NBP1-84449]
Immunohistochemistry-Paraffin: PLA2R1 Antibody [NBP1-84449] - Staining of human kidney shows weak membranous positivity in cells in glomeruli.![PLA2R1 Antibody - BSA Free Western Blot: PLA2R1 Antibody - BSA Free [NBP1-84449]](https://resources.rndsystems.com/images/products/nbp1-84449_rabbit-polyclonal-pla2r1-antibody-104202515421412.jpg "Western Blot: PLA2R1 Antibody - BSA Free [NBP1-84449]")
Western Blot: PLA2R1 Antibody - BSA Free [NBP1-84449]
Analysis in control (vector only transfected HEK293T lysate) and PLA2R1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY423477).Applications for PLA2R1 Antibody - BSA Free
Immunohistochemistry
Immunohistochemistry-Paraffin
Western Blot
Formulation, Preparation, and Storage
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Background: PLA2R1
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Additional PLA2R1 Products
Product Documents for PLA2R1 Antibody - BSA Free
Certificate of Analysis
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Product Specific Notices for PLA2R1 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
FAQs for PLA2R1 Antibody - BSA Free
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Q: How many test can be performed in 0.1 ml of PLA2R1 Antibody (NBP1-84449). Also kindly suggest the secondary Antibody for the same.
A: The number of tests using NBP1-84449 will depend upon the sample type (differential tissues expression) and the total volume of antibody used in each slide. This product is supplied in 100ul size and the concentration of the antibody is 0.4ug/ul and the working dilution we recommend for IHC is 1:500 to 1:1000, based on this information you should be able to estimate how many experiments can be done with one vial. As far as secondary antibody is concerned, any anti rabbit IgG secondary that is conjugated should technical work, the type of conjugation does not matter as much, HRP is most often used in labs while the biotin-conjugated antibody is used to amplify the signal which results in greater sensitivity than that achieved with an enzyme or fluorescence conjugated secondary antibody alone. Fluorescent labeled antibodies are commonly used for double or multiple staining methods. Here is the list of our secondary antibodies. I can recommend NB730-H as it has received very good reviews from our customers and it has been cited in 23 publications as well. I hope this answers your question. Please do not hesitate to contact us if you have any further questions.