Pladienolide B

Tocris Bioscience | Catalog # 6070

mRNA splicing inhibitor; antitumor
Tocris Bioscience

Key Product Details

Description

mRNA splicing inhibitor; antitumor
View all DNA, RNA and Protein Synthesis Inhibitors »

Product Description

Pladienolide B is a mRNA splicing inhibitor that decreases splicing capacity up to 75% in vitro. Pladienolide B directly targets splicesome-associated 130 (SAP130), inhibits splicing factor 3B subunit (SF3B1) and impairs U2 small nuclear ribonucleoprotein (U2 snRNP) interaction with pre-mRNA. Pladienolide B arrests the cell cycle in G1 and G2/M phases and displays antitumor activity against gastric cancer cells (IC50 values are 1.6-4.9 nM). Transient treatment with pladienolide B can reprogram pluripotent hESCs into a distinct type of zigotic genome activation (ZGA)-like cells (ZLCs). Cell permeable and active in vivo.

Product Specifications for Pladienolide B

Molecular Weight

536.70

Formula

C30H48O8

Storage

Store at -20°C

Purity

≥95% (HPLC)

Chemical Name

(4R,7R,8S,9E,11S,12S)-8-(Acetyloxy)-4,7-dihydroxy-12-[(1E,3E,5S)-6-[(2R,3R)-3-[(1R,2S)-2-hydroxy-1-methylbutyl]-2-oxiranyl]-1,5-dimethyl-1,3-hexadien-1-yl]-7,11-dimethyloxacyclododec-9-en-2-one

CAS Number

445493-23-2

PubChem ID

90909513

InChI Key

SDOUORKJIJYJNW-UHFFFAOYSA-N

SMILES

C[C@@H](/C=C/[C@H](OC(C)=O)[C@](C)(O)CC[C@@H](O)C1)[C@@H](/C(C)=C/C=C/[C@@H](C)C[C@@H]2[C@@H]([C@H](C)[C@@H](O)CC)O2)OC1=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 1 2

Preparing Stock Solutions for Pladienolide B

The following data is based on the product molecular weight 536.70.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.02 mM 93.16 mL 465.81 mL 931.62 mL
0.1 mM 18.63 mL 93.16 mL 186.32 mL
0.2 mM 9.32 mL 46.58 mL 93.16 mL
1 mM 1.86 mL 9.32 mL 18.63 mL

Calculators

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Background References

References are publications that support the biological activity of the product. See our Citations tab to view 27 publications citing the usage of this product.

Product Documents for Pladienolide B

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Pladienolide B

For research use only

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