PLAG1 Antibody (3B7) - Azide and BSA Free

Immunohistochemistry: PLAG1 Antibody (3B7) [H00005324-M02] -

Immunohistochemistry: PLAG1 Antibody (3B7) [H00005324-M02] - Detection of the FGFR1-PLAG1 & TGFBR3-PLAG1 fusion genes. a Illustration of the FGFR1-PLAG1 fusion gene. Arrows show locations of genomic break points for each of the tumors. b Expression of PLAG1 (left) & FGFR1 (right) in PLAG1 fusion-negative vs. FGFR1-PLAG1-positive tumors. Graphs show fragments per kilobase of exon per million fragments mapped (FPKM), based on RNA-seq data. ****P < 0.0001; Student’s t-test. n = 19 + 7. Horizontal lines show mean values. c Illustration of the TGFBR3-PLAG1 fusion gene. d Expression (FPKM) of PLAG1 (left) & TGFBR3 (right) in PLAG1 fusion-negative vs. TGFBR3-PLAG1-positive tumors. ***P < 0.001, ****P < 0.0001; Student’s t-test. n = 19 + 5. Horizontal lines show mean values. e Left, IHC showing TGFBR3 staining in whole tumor sections (upper panels, scale bars=5 mm) & enlargement of selected areas (lower panels, scale bars=100 μm). Right, quantification of IHC TGFBR3 levels in TGFBR3-PLAG1 positive vs. negative tumors. P (high TGFBR3) = 0.002, Fisher’s exact test. n = 5 + 34. f Poisson sample clustering based on gene expression in cohort 1, annotated by TGFBR3 IHC results Image collected & cropped by CiteAb from the following publication (https://www.nature.com/articles/s41467-017-01178-z), licensed under a CC-BY license. Not internally tested by Novus Biologicals.