Plastin L Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-19860

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Predicted:

Bovine (97%), Chicken (92%), Rat (97%), Rhesus Macaque (99%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Plastin L Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human Plastin L. The exact sequence is proprietary.

Reactivity Notes

Zebrafish (86%), Xenopus laevis (88%).

Localization

Cytoplasm

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

70 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Plastin L Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: Plastin L Antibody [NBP2-19860]

Immunocytochemistry/ Immunofluorescence: Plastin L Antibody [NBP2-19860]

Immunocytochemistry/Immunofluorescence: Plastin L Antibody [NBP2-19860] - Plastin L antibody detects Plastin L protein by immunofluorescent analysis. Sample: A549 cells were fixed in ice-cold MeOH for 5 min. Green: Plastin Lstained by Plastin L antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar= 10 um.
Western Blot: Plastin L Antibody [NBP2-19860]

Western Blot: Plastin L Antibody [NBP2-19860]

Western Blot: Plastin L Antibody [NBP2-19860] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Plastin L antibody diluted at 1:10000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Plastin L Antibody

Immunohistochemistry-Paraffin: Plastin L Antibody [NBP2-19860] -

Immunohistochemistry-Paraffin: Plastin L Antibody [NBP2-19860] - Plastin L antibody detects Plastin L protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded mouse thymus gland.
Plastin L stained by Plastin L antibody (NBP2-19860) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Plastin L Antibody - BSA Free

Western Blot: Plastin L Antibody [NBP2-19860] -

Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with L-Plastin antibody (NBP2-19860) diluted at 1:10000.
Plastin L Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: Plastin L Antibody [NBP2-19860] -

L-Plastin antibody detects L-Plastin protein at cytoplasm and focal adhesion site by immunofluorescent analysis.
Sample: A431 cells were fixed in ice-cold MeOH for 5 min.
Green: L-Plastin protein stained by L-Plastin antibody (NBP2-19860) diluted at 1:500.
Blue: Hoechst 33342 staining.
Plastin L Antibody - BSA Free

Western Blot: Plastin L Antibody [NBP2-19860] -

Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with L-Plastin antibody (NBP2-19860) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for Plastin L Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:5000-1:20000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 20% Glycerol

Format

BSA Free

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Plastin L

Plastins are a family of actin-binding proteins that are conserved throughout eukaryote evolution and expressed in most tissues of higher eukaryotes. In humans, two ubiquitous plastin isoforms (L and T) have been identified. Plastin 1 (otherwise known as Fimbrin) is a third distinct plastin isoform which is specifically expressed at high levels in the small intestine. The L isoform is expressed only in hemopoietic cell lineages, while the T isoform has been found in all other normal cells of solid tissues that have replicative potential (fibroblasts, endothelial cells, epithelial cells, melanocytes, etc.). However, L-plastin has been found in many types of malignant human cells of non-hemopoietic origin suggesting that its expression is induced accompanying tumorigenesis in solid tissues. [provided by RefSeq]

Alternate Names

CP64, DKFZp781A23186, FLJ25423, FLJ26114, FLJ39956, LC64PPLS2bA139H14.1 (lymphocyte cytosolic protein 1 (L-plastin)), LCP-1, LPL, L-plastin, L-plastin (Lymphocyte cytosolic protein 1) (LCP-1) (LC64P), Lymphocyte cytosolic protein 1, lymphocyte cytosolic protein 1 (L-plastin), Lymphocyte cytosolic protein-1 (plasmin), plastin 2, plastin-2

Gene Symbol

LCP1

UniProt

P13796 (Human)

Additional Plastin L Products

Product Documents for Plastin L Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Plastin L Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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