PLC-delta 3 Antibody

Novus Biologicals | Catalog # NBP3-20228

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (100%), Canine (100%), Mouse (100%), Porcine (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all PLC-delta 3 Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-HQYSGEDRVLSAPE, from the internal region of the protein sequence according to NP_588614.1.

Epitope

aa261-274

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Description

Novus Biologicals Goat PLC-delta 3 Antibody (NBP3-20228) is a polyclonal antibody validated for use in IHC, WB and ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for PLC-delta 3 Antibody

Immunohistochemistry-Paraffin: PLC-delta 3 Antibody [NBP3-20228]

Immunohistochemistry-Paraffin: PLC-delta 3 Antibody [NBP3-20228]

Immunohistochemistry-Paraffin: PLC-delta 3 Antibody [NBP3-20228] - Negative Control showing staining of paraffin embedded Human Testis, with no primary antibody.
Immunohistochemistry-Paraffin: PLC-delta 3 Antibody [NBP3-20228]

Immunohistochemistry-Paraffin: PLC-delta 3 Antibody [NBP3-20228]

Immunohistochemistry-Paraffin: PLC-delta 3 Antibody [NBP3-20228] - (6ug/ml) staining of paraffin embedded Human Testis. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
Immunohistochemistry-Paraffin: PLC-delta 3 Antibody [NBP3-20228]

Immunohistochemistry-Paraffin: PLC-delta 3 Antibody [NBP3-20228]

Immunohistochemistry-Paraffin: PLC-delta 3 Antibody [NBP3-20228] - Negative Control showing staining of paraffin embedded Human Kidney, with no primary antibody.
Immunohistochemistry-Paraffin: PLC-delta 3 Antibody [NBP3-20228]

Immunohistochemistry-Paraffin: PLC-delta 3 Antibody [NBP3-20228]

Immunohistochemistry-Paraffin: PLC-delta 3 Antibody [NBP3-20228] - (6ug/ml) staining of paraffin embedded Human Kidney. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
Western Blot: PLC-delta 3 Antibody [NBP3-20228]

Western Blot: PLC-delta 3 Antibody [NBP3-20228]

Western Blot: PLC-delta 3 Antibody [NBP3-20228] - (2ug/ml) staining of A549 (A) and Caco-2 (B) cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

Applications for PLC-delta 3 Antibody

Application
Recommended Usage

Immunohistochemistry-Paraffin

6 ug/ml

Peptide ELISA

Detection limit 1:32000

Western Blot

1-3 ug/ml
Application Notes
Western blot: Approx 85kDa band observed in lysates of cell limes A549 and Caco-2, and in preliminary testing of cell lines HeLa, HEK293 and U251 lysate (calculated MW of 89.3kDa according to NP_588614.1). Primary incubation 1 hour at room temperature.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PLC-delta 3

PLCD3, also referred to as Phospholipase C-delta-3, is 789 amino acids in length and approximately 89kDa. PLCD3 is a member of the Phospholipase C family and functions to hydrolyze PIP2 to produce the second messangers DAG and IP3. Current research on PLCD3 is being conducted in relation to several diseases and disorders including breast cancer, glioblastoma, Huntington's disease and neurological diseases. PLCD3 has also been shown to have interactions with ITPR1, ITPR3, PIK3C3, DGKE and SKIL in pathways such as the CREB pathway, sweet taste signaling and Inositol phosphate metabolism.

Long Name

Phospholipase C delta 3

Alternate Names

PLCD3, PLCdelta 3

Gene Symbol

PLCD3

Additional PLC-delta 3 Products

Product Documents for PLC-delta 3 Antibody

Certificate of Analysis

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Product Specific Notices for PLC-delta 3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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