PLK1 Antibody (13E8)

Novus Biologicals | Catalog # NB100-74502

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 13E8
View all PLK1 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein containing residues 300-400 of human PLK1.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Localization

Nuclear

Marker

Mitosis Marker

Specificity

Detects PLK1 from human, mouse, and rat samples.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for PLK1 Antibody (13E8)

Western Blot: PLK1 Antibody (13E8) [NB100-74502]

Western Blot: PLK1 Antibody (13E8) [NB100-74502]

Western Blot: PLK1 Antibody (13E8) [NB100-74502] - Proteins were transferred to a PVDF membrane and blocked with 5% Milk/TBST for at least 1 hour. Membranes were probed with PLK1 mouse monoclonal antibody at a dilution of 1:1000 overnight at 4C on a rocking platform. Membranes were washed in TBS-0.1%Tween 20 and probed with a goat anti-mouse-HRP secondary antibody at a dilution of 1:20,000 for at least one hour. Membranes were washed and chemiluminescent detection performed.
Immunocytochemistry/ Immunofluorescence: PLK1 Antibody (13E8) [NB100-74502]

Immunocytochemistry/ Immunofluorescence: PLK1 Antibody (13E8) [NB100-74502]

Immunocytochemistry/Immunofluorescence: PLK1 Antibody (13E8) [NB100-74502] - PLK1 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) in WiDr colon carcinoma cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing PLK1 at a dilution of 1:20 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.
Immunohistochemistry-Paraffin: PLK1 Antibody (13E8) [NB100-74502]

Immunohistochemistry-Paraffin: PLK1 Antibody (13E8) [NB100-74502]

Immunohistochemistry-Paraffin: PLK1 Antibody (13E8) [NB100-74502] - Both normal and cancer biopsies of deparaffinized human Colon tissue.
Immunocytochemistry/ Immunofluorescence: PLK1 Antibody (13E8) [NB100-74502]

Immunocytochemistry/ Immunofluorescence: PLK1 Antibody (13E8) [NB100-74502]

Immunocytochemistry/Immunofluorescence: PLK1 Antibody (13E8) [NB100-74502] - PLK1 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) in HeLa cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing PLK1 at a dilution of 1:20 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.
Immunocytochemistry/ Immunofluorescence: PLK1 Antibody (13E8) [NB100-74502]

Immunocytochemistry/ Immunofluorescence: PLK1 Antibody (13E8) [NB100-74502]

Immunocytochemistry/Immunofluorescence: PLK1 Antibody (13E8) [NB100-74502] - PLK1 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) in U251 glioma cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing PLK1 at a dilution of 1:20 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.

Applications for PLK1 Antibody (13E8)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:20

Immunohistochemistry

1:20

Immunohistochemistry-Paraffin

1:20

Immunoprecipitation

1:10 - 1:500

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS with 1 mg/ml BSA

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PLK1

Polo-like Kinase 1 (PLK1) is found to have several functions in mitotic progression. It also has been found to be elevated in a broad range of human tumors. The function of PLK1 appears to be a requirement for proper spindle assembly and other centrosome microtubule events.

Long Name

Polo-like Kinase 1

Alternate Names

STPK13

Entrez Gene IDs

5347 (Human); 18817 (Mouse); 25515 (Rat)

Gene Symbol

PLK1

UniProt

P53350

Additional PLK1 Products

Product Documents for PLK1 Antibody (13E8)

Certificate of Analysis

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Product Specific Notices for PLK1 Antibody (13E8)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Cancer Biomarkers
Intracellular Kinases

Citations for PLK1 Antibody (13E8)

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Protocols

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