PLK1 Antibody (2G12) - Azide and BSA Free

Novus Biologicals | Catalog # H00005347-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Proximity Ligation Assay

Cited:

Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 2G12

Format

Azide and BSA Free
View all PLK1 Primary Antibodies »

Product Specifications

Immunogen

PLK1 (AAH02369, 1 a.a. ~ 603 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MSAAVTAGKLARAPADPGKAGVPGVAAPGAPAAAPPAKEIPEVLVDPRSRRRYVRGRFLGKGGFAKCFEISDADTKEVFAGKIVPKSLLLKPHQREKMSMEISIHRSLAHQHVVGFHGFFEDNDFVFVVLELCRRRSLLELHKRRKALTEPEARYYLRQIVLGCQYLHRNRVIHRDLKLGNLFLNEDLEVKIGDFGLATKVEYDGERKKTLCGTPNYIAPEVLSKKGHSFEVDVWSIGCIMYTLLVGKPPFETSCLKETYLRIKKNEYSIPKHINPVAASLIQKMLQTDPTARPTINELLNDEFFTSGYIPARLPITCLTIPPRFSIAPSSLDPSNRKPLTVLNKGLENPLPERPREKEEPVVRETGEVVDCHLSDMLQQLHSVNASKPSERGLVRQEEAEDPACIPIFWVSKWVDYSDKYGLGYQLCDNSVGVLFNDSTRLILYNDGDSLQYIERDGTESYLTVSSHPNSLMKKITLLKYFRNYMSEHLLKAGANITPREGDELARLPYLRTWFRTRSAIILHLSNGSVQINFFQDHTKLILCPLMAAVTYIDEKRDFRTYRLSLLEEYGCCKELASRLRYARTMVDKLLSSRSASNRLKAS

Localization

Nuclear

Marker

Mitosis Marker

Specificity

PLK1 - polo-like kinase 1 (Drosophila)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for PLK1 Antibody (2G12) - Azide and BSA Free

Western Blot: PLK1 Antibody (2G12) [H00005347-M01]

Western Blot: PLK1 Antibody (2G12) [H00005347-M01]

Western Blot: PLK1 Antibody (2G12) [H00005347-M01] - Analysis of PLK1 expression in transfected 293T cell line by PLK1 monoclonal antibody (M01), clone 2G12.Lane 1: PLK1 transfected lysate(68.3 KDa).Lane 2: Non-transfected lysate.
Immunocytochemistry/ Immunofluorescence: PLK1 Antibody (2G12) [H00005347-M01]

Immunocytochemistry/ Immunofluorescence: PLK1 Antibody (2G12) [H00005347-M01]

Immunocytochemistry/Immunofluorescence: PLK1 Antibody (2G12) [H00005347-M01] - Analysis of monoclonal antibody to PLK1 on HeLa cell. Antibody concentration 10 ug/ml.
Western Blot: PLK1 Antibody (2G12) [H00005347-M01]

Western Blot: PLK1 Antibody (2G12) [H00005347-M01]

Western Blot: PLK1 Antibody (2G12) [H00005347-M01] - PLK1 monoclonal antibody (M01), clone 2G12 Analysis of PLK1 expression in Hela S3 NE.
Western Blot: PLK1 Antibody (2G12) [H00005347-M01]

Western Blot: PLK1 Antibody (2G12) [H00005347-M01]

Western Blot: PLK1 Antibody (2G12) [H00005347-M01] - Analysis of PLK1 in extracts from 293T, HeLa and A549 cell using anti-PLK1 monoclonal antibody.
Proximity Ligation Assay: PLK1 Antibody (2G12) [H00005347-M01]

Proximity Ligation Assay: PLK1 Antibody (2G12) [H00005347-M01]

Proximity Ligation Assay: PLK1 Antibody (2G12) [H00005347-M01] - Analysis of protein-protein interactions between CCNB1 and PLK1. Mahlavu cells were stained with anti-CCNB1 rabbit purified polyclonal 1:1200 and anti-PLK1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
Immunoprecipitation: PLK1 Antibody (2G12) [H00005347-M01]

Immunoprecipitation: PLK1 Antibody (2G12) [H00005347-M01]

Immunoprecipitation: PLK1 Antibody (2G12) [H00005347-M01] - Analysis of PLK1 transfected lysate using anti-PLK1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PLK1 MaxPab rabbit polyclonal antibody.
ELISA: PLK1 Antibody (2G12) [H00005347-M01]

ELISA: PLK1 Antibody (2G12) [H00005347-M01]

ELISA: PLK1 Antibody (2G12) [H00005347-M01] - Detection limit for recombinant GST tagged PLK1 is approximately 1ng/ml as a capture antibody.

Applications for PLK1 Antibody (2G12) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Immunoprecipitation

1:10-1:500

Western Blot

1:500
Application Notes
This antibody reactivity against cell lysate and recombinant protein for WB. It has been useful for IF and ELISA. IHC-P usage reported in scientific literature (PMID: 24722282).

Formulation, Preparation, and Storage

Purification

Ascites

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: PLK1

PLK1 (polo-like kinase 1) is a member of the serine/threonine protein kinase family, cdc5/polo subfamily. PLK1 contains two polo box domains with a predicted molecular weight of 68 kDa. PLK1 has been shown to regulate cdc2/cyclin B through phosphorylation and activation of cdc25c phosphatase. PLK1 is modified by phosphorylation at Threonine 210. PLK1 may also be required for cell division, depletion of PLK1 results in apoptosis and deregulation of expression of PKL1 is correlated with development of many malignancies.

Long Name

Polo-like Kinase 1

Alternate Names

STPK13

Entrez Gene IDs

5347 (Human)

Gene Symbol

PLK1

OMIM

602098 (Human)

UniProt

AAH02369 (Human)

Additional PLK1 Products

Product Documents for PLK1 Antibody (2G12) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for PLK1 Antibody (2G12) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Cancer Biomarkers
Intracellular Kinases

Citations for PLK1 Antibody (2G12) - Azide and BSA Free

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Protocols

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FAQs

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