PLK1 [p Thr210] Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-78021

Novus Biologicals
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all PLK1 Primary Antibodies »

Product Specifications

Immunogen

PLK1 [p Thr210] Antibody was produced by repeated immunizations with a synthetic phospho peptide corresponding to an internal region near aa 200-225 of Human Polo-like kinase 1 (Plk1) protein. (Uniprot: P53350)

Reactivity Notes

BLAST analysis indicates 100 % homology of the immunizing sequence with PLK1 homologues from human, chimpanzee, pig, chicken, mouse, rat, Xenopus, dog, mosquito, zebra fish, starfish, sea urchin and fruit fly. Cross reactivity with PLK1 protein homologues from C.elgans and honeybee may also occur as sequence homology varies by one amino acid residue in this sequence. Reactivity with PLK1 protein from other sources is not known. Minimal reactivity is expected with the non-phosphorylated form of the protein.

Modification

p Thr210

Marker

Mitosis Marker

Specificity

Anti-Polo-like Kinase pT210 Antibody is directed against human phosphorylated Plk1 protein. This antibody is specific for phosphorylated human PLK1 protein at the pT210 residue. BLAST analysis indicates 100 % homology of the immunizing sequence with PLK1 homologues from human, chimpanzee, pig, chicken, mouse, rat, Xenopus, dog, mosquito, zebra fish, starfish, sea urchin and fruit fly. Cross reactivity with PLK1 protein homologues from C.elgans and honeybee may also occur as sequence homology varies by one amino acid residue in this sequence. Reactivity with PLK1 protein from other sources is not known. Minimal reactivity is expected with the non-phosphorylated form of the protein.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody is directed against human phosphorylated Plk1 protein. This antibody was affinity purified

Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Scientific Data Images for PLK1 [p Thr210] Antibody - BSA Free

Western Blot: PLK1 [p Thr210] Antibody [NBP1-78021]

Western Blot: PLK1 [p Thr210] Antibody [NBP1-78021]

Western Blot: PLK1 [p Thr210] Antibody [NBP1-78021] - Western blot analysis is shown to detect endogenous and recombinant protein present in HeLa cell lysates transfected with various plk-1 mutation constructs. Blots were reacted with anti-Plk-1 pT210 (panel A) and pan reactive anti-Plk-1 (panel B). Transfected cells were treated with 1 uM nocodazole followed by cell collection, lysate preparation, SDS-PAGE and western blotting. Using a 1:1000 dilution, anti-Plk-1 pT210 detects a single band corresponding to endogenous plk-1, but does not detect recombinant forms of the protein presumably because of a lack of phosphorylation in these mutants.
Immunohistochemistry: PLK1 [p Thr210] Antibody [NBP1-78021]

Immunohistochemistry: PLK1 [p Thr210] Antibody [NBP1-78021]

Immunohistochemistry: PLK1 [p Thr210] Antibody [NBP1-78021] - Used at a 1:200 dilution to detect Plk1 by immunohistochemistry in human breast carcinoma tumor tissue. Tissue was formalin-fixed and paraffin embedded.
Western Blot: PLK1 [p Thr210] Antibody [NBP1-78021]

Western Blot: PLK1 [p Thr210] Antibody [NBP1-78021]

Western Blot: PLK1 [p Thr210] Antibody [NBP1-78021] - Plk-1 pT210 antibody to detect endogenous protein present in a Mouse A20 whole cell lysate (arrowhead). Comparison to a molecular weight marker indicates a band of ~68 kDa corresponding to Plk-1 protein. It is suggested to use a nuclear extract from synchronized cells to greatly increase the abundance of this protein in preparations. The blot was incubated with a 1:500 dilution of the antibody at room temperature followed by detection using standard techniques.
Immunohistochemistry: PLK1 [p Thr210] Antibody [NBP1-78021]

Immunohistochemistry: PLK1 [p Thr210] Antibody [NBP1-78021]

Immunohistochemistry: PLK1 [p Thr210] Antibody [NBP1-78021] - Used at a 1:200 dilution to detect Plk1 by immunohistochemistry in human colon carcinoma tumor tissue. Tissue was formalin-fixed and paraffin embedded.
PLK1 [p Thr210] Antibody

PLK1 [p Thr210] Antibody

Affinity Purified Plk1 pT210 was used at a 1:200 dilution to detect Plk1 by immunohistochemistry in human breast carcinoma tumor tissue. Tissue was formalin-fixed and paraffin embedded. Personal Communication, Alan Yen, LifeSpanBiosciences, Seattle, WA.
PLK1 [p Thr210] Antibody

PLK1 [p Thr210] Antibody

Western blot analysis is shown to detect endogenous and recombinant protein present in HeLa cell lysates transfected with various plk-1 mutation constructs. Blots were reacted with aT210 (panel A) and pan reactive apanel B). Transfected cells were treated with 1 uM nocodazole followed by cell collection, lysate preparation, SDS-PAGE and western blotting. Using a 1:1000 dilution, aT210 detects a single band corresponding to endogenous plk-1, but does not detect recombinant forms of the protein presumably because of a lack of phosphorylation in these mutants. Personal communication Hai Jiang, Northwestern Univ.

Applications for PLK1 [p Thr210] Antibody - BSA Free

Application
Recommended Usage

ELISA

1:3000-1:12000

Immunohistochemistry

1:200-1:1000

Immunohistochemistry-Paraffin

1:200

Western Blot

1:200-1:2000
Application Notes
This affinity-purified antibody has been tested for use in ELISA, IHC, and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 68 kDa in size corresponding to Plk-1 by western blotting in the appropriate cell lysate or extract. This antibody is positive by IHC on kidney, liver, cancer, thyroid and lymphocyte tissue.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Format

BSA Free

Preservative

0.01% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.

Background: PLK1

Polo-like kinase 1, also known as Plk-1, serine-threonine protein kinase 13 and STPK13, may be required for cell division and have a role during G1 or S phase and is associated with the phosphorylation of cyclin B1. Phosphorylation of Plk-1 on T210 contributes to proper mitotic progression. Plk-1 has a nuclear localization and accumulates to a maximum during the G2 and M phases, declines to a nearly undetectable level following mitosis and throughout G1 phase, and then begins to accumulate again during S phase. Plk-1 is a member of the Ser/Thr protein kinase family and the CDC5/Polo subfamily and contains 2 POLO box domains.

Long Name

Polo-like Kinase 1

Alternate Names

STPK13

Entrez Gene IDs

5347 (Human)

Gene Symbol

PLK1

UniProt

P53350

Additional PLK1 Products

Product Documents for PLK1 [p Thr210] Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for PLK1 [p Thr210] Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Cancer Biomarkers
Intracellular Kinases

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